APCs, BCA, CD,Enteric, GST–GFP, IPTG, immobilisation, LP, MALDI-TOF, MDA, PP, Peyer’s patch, SANS, SDS PAGE, silica, synthetic protein, VDS, vaccine, antigen presenting cells, bicinchoninic acid assay, circular dichroism, glutathione-S-transferase (GST), lamina propria, maternally derived antibodies, matrix assisted laser desorption ionisation time o, small angle neutron scattering, sodium dodecyl sulphate polyacrylamide gel electro, vaccine delivery systemsenteric, immobilisation

Pettit, Marie, Dyer, Paul D.R., Mitchell, John C. ORCID: 0000-0003-2945-3292 , Griffiths, Peter C. ORCID: 0000-0002-6686-1271 , Alexander, Bruce, Cattoz, Beatrice, Heenan, Richard K., King, Stephen M., Schweins, Ralf, Pullen, Frank, Wicks, Stephen R. and Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 (2014) Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS). International Journal of Pharmaceutics, 468 (1-2). pp. 264-271. ISSN 0378-5173 (doi:https://doi.org/10.1016/j.ijpharm.2014.03.046)

Alginate dressing, GST-GFP Proteins, Wound healing

Boateng, Joshua ORCID: 0000-0002-6310-729X , Momoh, Frederick, Richardson, Simon ORCID: 0000-0002-7927-0649 , Chowdhry, Babur and Mitchell, John ORCID: 0000-0003-2945-3292 (2015) Development functional characterization of alginate dressing as potential protein delivery system for wound healing. International Journal of Biological Macromolecules, 81. pp. 137-150. ISSN 0141-8130 (Print), 1879-0003 (Online) (doi:https://doi.org/10.1016/j.ijbiomac.2015.07.037)

Antisense; Anthrax toxin; siRNA; Endocytosis; Cell biology; RNAi; PEG-dilemma; Non-viral

Dyer, Paul D.R., Shepherd, Thomas R., Gollings, Alexander S., Shorter, Susan A., Gorringe-Pattrick, Monique A. M., Tang, Chun-Kit, Cattoz, Beatrice N., Baillie, Les, Griffiths, Peter C. ORCID: 0000-0002-6686-1271 and Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 (2015) Disarmed anthrax toxin delivers antisense oligonucleotides and siRNA with high efficiency and low toxicity. Journal of Controlled Release, 220 (A). pp. 316-328. ISSN 0168-3659 (doi:https://doi.org/10.1016/j.jconrel.2015.10.054)

Antisense; Drug Delivery; Endocytosis; Gene Therapy; siRNA; Toxin

Shorter, Susan, Gollings, Alexander, Gorringe-Pattrick, Monique, Coakley, Emma, Dyer, Paul D.R. and Richardson, Simon ORCID: 0000-0002-7927-0649 (2016) The potential of toxin-based drug delivery systems for enhanced nucleic acid therapeutic delivery. Expert Opinion on Drug Delivery, 14 (5). pp. 685-696. ISSN 1742-5247 (Print), 1744-7593 (Online) (doi:https://doi.org/10.1080/17425247.2016.1227781)

Cassava, whitefly, Arsenophonus, Rickettsia, fitness costs, virus retention

Ghosh, Saptarshi, Bouvaine, Sophie ORCID: 0000-0002-0788-3243 , Richardson, Simon C. W. ORCID: 0000-0002-7927-0649 , Ghanim, Murad and Maruthi, M.N. ORCID: 0000-0002-8060-866X (2017) Fitness costs associated with infections of secondary endosymbionts in the cassava whitefly species Bemisia tabaci. Journal of Pest Science, 91. pp. 17-28. ISSN 1612-4758 (Print), 1612-4766 (Online) (doi:https://doi.org/10.1007/s10340-017-0910-8)

GFP, Marker, Endocytosis, Protein stability, Protease, Drug delivery, Membrane

Shorter, Susan, Pettit, Marie W., Dyer, Paul D.R., Coakley-Youngs, J. Emma, Gorringe-Pattrick, Monique A. M., El-Daher, Samer and Richardson, Simon ORCID: 0000-0002-7927-0649 (2017) Green Fluorescent Protein (GFP): is seeing believing and is that enough? Journal of Drug Targeting, 25 (9-10). pp. 809-817. ISSN 1061-186X (Print), 1029-2330 (Online) (doi:https://doi.org/10.1080/1061186X.2017.1358725)

In vitro model, Spreading, Leishmania aethiopica, Leishmania mexicana, Human monocytes, Infection, THP-1, Retinoic acid

Rai, Rajeev, Dyer, Paul, Richardson, Simon ORCID: 0000-0002-7927-0649 , Harbige, Laurence and Getti, Giulia ORCID: 0000-0003-1402-8496 (2017) Apoptotic induction induces Leishmania aethiopica and L. mexicana spreading in terminally differentiated THP-1 cells. Parasitology, 144 (14). pp. 1912-1921. ISSN 0031-1820 (Print), 1469-8161 (Online) (doi:https://doi.org/10.1017/S0031182017001366)

Polymers, Microgel, Fluorescence

Mohsen, R., Alexander, B. D., Richardson, S. C. W. ORCID: 0000-0002-7927-0649 , Mitchell, J. C. ORCID: 0000-0003-2945-3292 , Diab, A. A. and Snowden, M. J. ORCID: 0000-0002-1087-2692 (2016) Design, synthesis, characterization and toxicity studies of Poly (N-Iso-Propylacrylamide-co-Lucifer Yellow) particles for drug delivery applications. Journal of Nanomedicine and Nanotechnology, 7 (2):1000363. pp. 363-372. ISSN 2157-7439 (doi:https://doi.org/10.4172/2157-7439.1000363)

Ricin toxin; Endocytosis; Polyphenol; Epigallocatechin gallate; eGCG; Tea

Dyer, Paul D. R., Kotha, Arun K., Gollings, Alex S., Shorter, Susan A., Shepherd, Thomas R., Pettit, Marie W., Alexander, Bruce D., Getti, Giulia T. M. ORCID: 0000-0003-1402-8496 , El-Daher, Samer, Baillie, Les and Richardson, Simon C. W. ORCID: 0000-0002-7927-0649 (2016) An in vitro evaluation of epigallocatechin gallate (eGCG) as a biocompatible inhibitor of ricin toxin. Biochimica et Biophysica Acta (BBA) - General Subjects, 1860 (7). pp. 1541-1550. ISSN 0304-4165 (doi:https://doi.org/10.1016/j.bbagen.2016.03.024)

Ruth Duncan, Drug Delivery, Polymers, Polymer Therapeutics

Satchi-Fainaro, Ronit, Vicent, Maria and Richardson, Simon ORCID: 0000-0002-7927-0649 (2017) Professor Ruth Duncan: a pioneer in the field of polymer therapeutics. Journal of Drug Targeting, 25 (9-10). pp. 757-758. ISSN 1061-186X (Print), 1029-2330 (Online) (doi:https://doi.org/10.1080/1061186X.2017.1371722)

antisense oligonucleotide; ASO; cytosolic delivery

Richardson, Dyer, Mitchell (2014) WO 2014203008 A1 Antisense oligonucleotide compositions. WO2014203008 A1.

antisense; siRNA; toxin; cytosol; endocytosis; exosomes; biologics

Feron, Benedita and Richardson, Simon ORCID: 0000-0002-7927-0649 (2022) Engineering protein toxins to modulate the intracellular trafficking of biologics into exosomes for third order drug targeting. European Journal of Extracellular Vesicles, 2. pp. 1-14. ISSN 2001-3078

apoptosis, axenic amastigotes, L. aethiopica, L. mexicana, infection spreading, GFP, THP-1, trafficking, EEA1 (Early Endosome Antigen 1), LAMP-1 (lysosomal-associated membrane protein 1)

Ranatunga, Medhavi, Rai, Rajeev, Richardson, Simon C. W. ORCID: 0000-0002-7927-0649 , Dyer, Paul, Harbige, Laurence, Deacon, Andrew ORCID: 0000-0001-7630-4973 , Pecorino, Lauren and Getti, Giulia T. M. ORCID: 0000-0003-1402-8496 (2019) Leishmania aethiopica cell-to-cell spreading involves caspase-3, AkT and NF-jB but not PKC-d activation and involves uptake of LAMP-1 positive bodies containing parasites. The FEBS Journal (Federation of European Biochemical Societies), 287 (9). pp. 1777-1797. ISSN 1742-464X (Print), 1742-4658 (Online) (doi:https://doi.org/10.1111/febs.15166)

autism; human neural progenitors; stem cells; CHD8

Coakley-Youngs, Emma, Ranatunga, Medhavi, Richardson, Simon ORCID: 0000-0002-7927-0649 , Getti, Giulia ORCID: 0000-0003-1402-8496 , Shorter, Susan and Fivaz, Marc ORCID: 0000-0003-1003-7934 (2022) Autism-associated CHD8 keeps proliferation of human neural progenitors in check by lengthening the G1 phase of the cell cycle. Biology Open, 11 (9):bio058941. pp. 1-11. ISSN 2046-6390 (Online) (doi:https://doi.org/10.1242/bio.058941)

drug delivery, gene therapy, polymers, nanomedicine, cytotoxicity, poly(amido-amine), poly(ethyleneimine), polyamidoamines, poly-L-lysine, poor drug solubility

Fenili, Fabio, Ferrari, Elena, Manfredi, Amedea, Cavalli, Roberta, Richardson, Simon ORCID: 0000-0002-7927-0649 , Ranucci, Elisabetta and Ferruti, Paolo (2012) Poly(amido-amine)s as carriers for intracellular delivery of drugs and proteins. In: Sebastian, Mathew, Ninan, Neetha and Haghi, A.K., (eds.) Nanomedicine and Drug Delivery. Advances in Nanoscience and Nanotechnology (v.1). Apple Academic Press Inc., Toronto, Canada & New Jersey, USA., pp. 51-64. ISBN 978-1-926895-17-8 (hardback), 978-1-46-656007-9 (e-book)

drug delivery, polymers, cell biology

Pettit, Marie W., Griffiths, Peter ORCID: 0000-0002-6686-1271 , Ferruti, Paolo and Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 (2011) Poly(amidoamine) polymers: soluble linear amphiphilic drug-delivery systems for genes, proteins and oligonucleotides. Therapeutic Delivery, 2 (7). pp. 907-917. ISSN 2041-5990 (doi:https://doi.org/10.4155/tde.11.55)

endocytosis, cell biology, Fab1, PIKfyve, PtdInsI(3,5)P2, spongiform, endosomal traffic

Zhang, Yanling, Zolov, Sergey N., Chow, Clement Y., Slutsky, Shalom G., Richardson, Simon C. ORCID: 0000-0002-7927-0649 , Piper, Robert C., Yang, Baoli, Nau, Johnathan J., Westrick, Randal J., Morrison, Sean J., Meisler, Miriam H. and Weisman, Lois S. (2007) Loss of Vac14, a regulator of the signaling lipid phosphatidylinositol 3,5-bisphosphate, results in neurodegeneration in mice. PNAS, 104 (44). pp. 17518-17523. ISSN 1091-6490 (doi:https://doi.org/10.1073/pnas.0702275104)

endocytosis, drug delivery, organelle, fluorescent microscopy, live-cell imaging, polymers

Dyer, Paul D.R., Kotha, Arun K., Pettit, Marie W. and Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 (2013) Imaging select mammalian organelles using fluorescent microscopy: application to drug delivery. In: Weissig, Volkmar, Elbayoumi, Tamer and Olsen, Mark, (eds.) Methods in Molecular Biology: Cellular and subcellular nanotechnology. Methods in Molecular Biology, 991 . Humana Press, New York, USA, pp. 195-210. ISBN 9781627033350 (doi:https://doi.org/10.1007/978-1-62703-336-7)

endocytosis, endomembrane, poly(amidoamine), toxin, trafficking

Dyer, Paul D.R. and Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 (2011) Delivery of biologics to select organelles – the role of biologically active polymers. Expert Opinion on Drug Delivery, 8 (4). pp. 403-407. ISSN 1742-5247 (doi:https://doi.org/10.1517/17425247.2011.558080)

endocytosis, nanomedicine, endosome, lysosome, trafficking, lysosomotropic delivery, endosomotropic delivery

Duncan, Ruth and Richardson, Simon ORCID: 0000-0002-7927-0649 (2012) Endocytosis and intracellular trafficking as gateways for nanomedicine delivery: opportunities and challenges. Molecular Pharmaceutics, 9 (9). pp. 2380-2402. ISSN 1543-8384 (Print), 1543-8392 (Online) (doi:https://doi.org/10.1021/mp300293n)

endocytosis, polymer conjugates, nanomedicine, intracellular trafficking

Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 , Wallom, Kerri-Lee, Ferguson, Elaine L., Deacon, Samuel P.E., Davies, Matthew W., Powell, Alison J., Piper, Robert C. and Duncan, Ruth (2008) The use of fluorescence microscopy to define polymer localisation to the late endocytic compartments in cells that are targets for drug delivery. Journal of Controlled Release, 127 (1). pp. 1-11. ISSN 0168-3659 (doi:https://doi.org/10.1016/j.jconrel.2007.12.015)

endosomolytic polymer, poly(amidoamine), subcellular fractionation

Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 , Pattrick, Nicola G., Lavignac, Nathalie, Ferruti, Paolo and Duncan, Ruth (2009) Intracellular fate of bioresponsive poly(amidoamine)s in vitro and in vivo. Journal of Controlled Release, 142 (1). pp. 78-88. ISSN 0168-3659 (doi:https://doi.org/10.1016/j.jconrel.2009.09.025)

estrogen-receptor modulators, tyrosine kinase-activity, breast-cancer, in-vitro, inhibitors, antimalarial, chloroquine, ferrocifens, activation, chemistry

Spencer, John, Mendham, Andrew P., Kotha, Arun K., Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 , Hillard, Elizabeth A., Jaouen, Gérard, Male, Louise and Hursthouse, Michael B. (2008) Structural and biological investigation of ferrocene-substituted 3-methylidene-1,3-dihydro-2H-indol-2-ones. Dalton Transactions, 2009 (6). pp. 918-921. ISSN 1477-9226 (Print), 1477-9234 (Online) (doi:https://doi.org/10.1039/B816249B)

exocytosis, lysosomes, nucleus, tumor viruses, viral replication, Merkel cell polyomavirus, T Antigen, hVam6p, nuclear sequestration, polyomavirus

Liu, Xi, Hein, Jennifer, Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 , Basse, Per H., Toptan, Tuna, Moore, Patrick S., Gjoerup, Ole V. and Chang, Yuan (2011) Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus. The Journal of Biological Chemistry, 286 (19). pp. 17079-17090. ISSN 0021-9258 (Print), 1083-351X (Online) (doi:https://doi.org/10.1074/jbc.M110.192856)

gene delivery, layer-by-layer assembly, nanoparticles, self-assembly, zeta-potential

Griffiths, Peter C. ORCID: 0000-0002-6686-1271 , Mauro, Nicolo, Murphy, Damien M., Carter, Emma, Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 , Dyer, Paul and Ferruti, Paolo (2013) Self-assembled PAA-based nanoparticles as potential gene and protein delivery systems. Macromolecular Bioscience, 13 (5). pp. 641-649. ISSN 1616-5187 (Print), 1616-5195 (Online) (doi:https://doi.org/10.1002/mabi.201200462)

intracellular polymer localization, fluorescence microscopy

Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 (2010) Tracking intracellular polymer localization via fluorescence microscopy. In: Weissig, Volkmar and D'Souza, Gerard G.M., (eds.) Organelle-Specific Pharmaceutical Nanotechnology. John Wiley & Sons, Inc., pp. 177-192. ISBN 9780470631652

organometallic chemistry, antitumor-activity, complexes, drugs, precatalysts, inhibitors, palladium, design, agents

Spencer, John, Rathnam, Rajendra P., Montukuri, Mahesh, Kotha, Arun K., Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 , Hazrati, Ali, Hartley, John A., Male, Louise and Hursthouse, Michael B. (2009) Synthesis of a 1,4-benzodiazepine containing palladacycle with in vitro anticancer and cathepsin B activity. Dalton Transactions (22). pp. 4299-4303. ISSN 1477-9226 (Print), 1477-9234 (Online) (doi:https://doi.org/10.1039/b819061e)

polymer therapeutics, cancer, PLA2, dextrin, anticancer conjugates, nanomedicines, PUMPT

Ferguson, Elaine L., Richardson, Simon C.W. ORCID: 0000-0002-7927-0649 and Duncan, Ruth (2010) Studies on the mechanism of action of dextrin−phospholipase A₂and its suitability for use in combination therapy. Molecular Pharmaceutics, 7 (2). pp. 510-521. ISSN 1543-8384 (Print), 1543-8392 (Online) (doi:https://doi.org/10.1021/mp900232a)

siRNA, toxins, antisense, endocytosis, membraine

Feron, Benedita Kaç Labbé and Richardson, Simon Clifford Wainwright ORCID: 0000-0002-7927-0649 (2018) The delivery of personalised, precision medicines via synthetic proteins. Drug Delivery Letters, 9 (1). pp. 79-88. ISSN 2210-3031 (Print), 2210-304X (Online) (doi:https://doi.org/10.2174/2210303109666181224115722)