Alberini, Isabella, Del Tordello, Elena, Fasolo, Alba, Temperton, Nigel J. ORCID: https://orcid.org/0000-0002-7978-3815, Galli, Grazia, Gentile, Chiara, Montomoli, Emanuele, Hilbert, Anne K., Banzhoff, Angelika, Del Giudice, Giuseppe, Donnelly, John J., Rappuoli, Rino and Capecchi, Barbara (2009) Pseudoparticle neutralization is a reliable assay to measure immunity and cross-reactivity to H5N1 influenza viruses. Vaccine, 27 (43). pp. 5998-6003. ISSN 0264-410X (doi:10.1016/j.vaccine.2009.07.079)

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Abstract

The standard serological methods present limitations for the measurement of immunity against H5N1 influenza strains. The hemagglutination inhibition (HI) assay lacks sensitivity and requires standardization, while the viral micro-neutralization (MN) assay needs handling of live virus. We produced pseudoparticles expressing hemagglutinin from clades 1 or 2 H5N1 in order to measure neutralizing antibodies in human sera after prime-boost vaccination with plain or MF59-adjuvanted H5N1 clade 1 subunit vaccines. Titers measured by pseudoparticle neutralization (PPN) assay significantly correlated with those measured by HI, single radial haemolysis or MN, with a PPN titer of 1:357 corresponding to an MN titer of 1:80. Notably, results from the PPN assay, confirm that MF59-H5N1 vaccine induces potent and long-lasting neutralizing antibody responses not only against the vaccine strain, but also against several heterologous clade 2 strains. Overall, the PPN assay represents a valid alternative to conventional serological methods for the evaluation of H5N1 vaccine immunogenicity.

Item Type:	Article
Uncontrolled Keywords:	pseudoparticle neutralization assay, avian influenza virus, H5N1, pandemic vaccine, cross-clade neutralization, serological methods
Subjects:	R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine R Medicine > RC Internal medicine
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science > Medway School of Pharmacy
Related URLs:	Publisher Organisation
Last Modified:	13 Jul 2012 13:36
URI:	http://gala.gre.ac.uk/id/eprint/8553

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