Scott, S.D. and Marples, B. (2000) Comment on the use of the cre/loxP recombinase system for gene therapy vectors. Gene Therapy, 7 (19). p. 1706. ISSN 0969-7128 (Print), 1476-5462 (Online) (doi:https://doi.org/10.1038/sj.gt.3301305)

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Abstract

In the recent editorial 'Radiation to control gene expression', Stackhouse and Buchsbaum1 gave an excellent summary of the published research on the use of radiation to target gene therapy for cancer. They also comment on several issues that will have to be addressed before this approach can have a practical application. In the article, they outline our own work2 on a 'molecular switch' to enhance therapeutic gene expression following a comparatively low induction dose of radiation. Commenting on the use of the cre/loxP recombination system, they state: 'The hypothesis that once cre recombinase is produced it will continue to create a functional therapeutic transcript has not been tested. The durability of expression of the cre gene and the stability of the cre enzyme will be critical determinants for the continued expression of the therapeutic gene'. In fact, the basis of the system we describe obviates the need for the continued expression of cre via the radiation-responsive promoter. Consequently, a brief review of the mechanism we have utilised is appropriate to clarify the situation.

Item Type:	Article
Additional Information:	[1] This is a letter to the Gene Therapy journal commenting on an editorial entitled 'Radiation to control gene expression' which was published in Gene Therapy July 2000, Volume 7, Number 13, Pages 1085-1086. doi:10.1038/sj.gt.3301233
Uncontrolled Keywords:	gene therapy, cancer, cre/loxP, CMV promoter, DNA, molecular switch
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) T Technology > TP Chemical technology
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science > Medway School of Pharmacy Faculty of Engineering & Science
Related URLs:	Publisher
Last Modified:	14 Jul 2015 15:53
URI:	http://gala.gre.ac.uk/id/eprint/8475

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