Corti, Davide, Voss, Jarrod, Gamblin, Steven J., Codoni, Giosiana, Macagno, Annalisa, Jarrossay, David, Vachieri, Sebastien G., Pinna, Debora, Minola, Andrea, Vanzetta, Fabrizia, Silacci, Chiara, Fernandez-Rodriguez, Blanca M., Agatic, Gloria, Bianchi, Siro, Giacchetto-Sasselli, Isabella, Calder, Lesley, Sallusto, Federica, Collins, Patrick, Haire, Lesley F., Temperton, Nigel ORCID: 0000-0002-7978-3815 , Langedijk, Johannes P.M., Skehel, John J. and Lanzavecchia, Antonio (2011) A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins. Science, 333 (6044). pp. 850-856. ISSN 0036-8075 (Print), 1095-9203 (Online) (doi:https://doi.org/10.1126/science.1205669)

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Abstract

The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency.

Item Type:	Article
Uncontrolled Keywords:	neutralizing antibody, plasma cells, group 1, group 2, influenza A virus, hemagglutinins
Subjects:	R Medicine > RS Pharmacy and materia medica
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science > Medway School of Pharmacy Faculty of Engineering & Science
Related URLs:	Publisher
Last Modified:	27 Jul 2015 11:52
URI:	http://gala.gre.ac.uk/id/eprint/8421

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