Abu-Rabie, Paul, Denniff, Philip, Spooner, Neil, Brynjolffssen, Joyce, Galluzzo, Paul and Sanders, Giles (2011) Method of applying internal standard to dried matrix spot samples for use in quantitative bioanalysis. Analytical Chemistry (including News & Features), 83 (22). pp. 8779-8786. ISSN 1520-6882 (online) (doi:10.1021/ac202321q)

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Abstract

A novel technique is presented that addresses the issue of how to apply internal standard (IS) to dried matrix spot (DMS) samples that allows the IS to integrate with the sample prior to extraction. The TouchSpray, a piezo electric spray system, from The Technology Partnership (TTP), was used to apply methanol containing IS to dried blood spot (DBS) samples. It is demonstrated that this method of IS application has the potential to work in practice, for use in quantitative determination of circulating exposures of pharmaceuticals in toxicokinetic and pharmacokinetic studies. Three different methods of IS application were compared: addition of IS to control blood prior to DBS sample preparation (control 1), incorporation into extraction solvent (control 2), and the novel use of TouchSpray technology (test). It is demonstrated that there was no significant difference in accuracy and precision data using these three techniques obtained using both manual extraction and direct elution.

Item Type:	Article
Uncontrolled Keywords:	to dried matrix spot(DMS), extraction, piezo electric spray system, toxicokinetic, pharmacokinetic
Subjects:	R Medicine > RM Therapeutics. Pharmacology T Technology > T Technology (General)
Pre-2014 Departments:	School of Science
Related URLs:	Publisher Publisher Organisation
Last Modified:	14 Oct 2016 09:18
URI:	http://gala.gre.ac.uk/id/eprint/7379

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