Zhu, Zhiqiang, Yang, Chenjie, Keyhani, Nemat O., Liu, Sen, Pu, Huili, Jia, Peisong, Wu, Dongmei, Stevenson, Philip C. ORCID: 0000-0002-0736-3619 , Fernandez-Grandon, G. Mandela ORCID: 0000-0002-2993-390X , Pan, Jieming, Chen, Yuxi, Guan, Xiayu and Qiu, Junzhi (2023) Characterization of Terpenoids from the Ambrosia Beetle Symbiont and Laurel Wilt Pathogen Harringtonia lauricola. Journal of Fungi, 9 (12):1175. ISSN 2309-608X (Online) (doi:https://doi.org/10.3390/jof9121175)

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Abstract

Little is known concerning terpenoids produced by members of the fungal order Ophiostomales, with the member Harringtonia lauricola having the unique lifestyle of being a beetle symbiont but potentially devastating tree pathogen. Nine known terpenoids, including six labdane diterpenoids (1–6) and three hopane triterpenes (7–9), were isolated from H. lauricola ethyl acetate (EtOAc) ex-tracts for the first time. All compounds were tested for various in vitro bioactivities. Six com-pounds, 2, 4, 5, 6, 7, and 9, are described functionally. Compounds 2, 4, 5, and 9 expressed potent antiproliferative activity against the MCF-7, HepG2 and A549 cancer cell lines, with half-maximal inhibitory concentrations (IC50s) ~12.54–26.06 μM. Antimicrobial activity bioas-says revealed that compounds 4, 5, and 9 exhibited substantial effects against Gram-negative bac-teria (Escherichia coli and Ralstonia solanacearum) with minimum inhibitory concentration (MIC) values between 3.13 and 12.50 μg/mL. Little activity was seen towards Gram-positive bacteria for any of the compounds, whereas compounds 2, 4, 7, and 9 expressed antifungal activities (Fusarium oxysporum) with MIC values ranging from 6.25 to 25.00 μg/mL. Compounds 4, 5, and 9 also dis-played free radical scavenging abilities towards 2,2-diphenyl-1-picrylhydrazyl (DPPH) and su-peroxide (O2−), with IC50 values of compounds 2, 4, and 6 ~3.45–14.04 μg/mL and 22.87–53.31 μg/mL towards DPPH and O2−, respectively. These data provide an insight into the biopharmaceu-tical potential of terpenoids from this group of fungal insect symbionts and plant pathogens.

Item Type:	Article
Uncontrolled Keywords:	Ophiostomales; Harringtonia lauricola; laurel wilt; secondary metabolite; terpenoid; antimicrobial; antiproliferative; free radical scavenging
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Medway School of Pharmacy Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified:	09 Jan 2024 10:28
URI:	http://gala.gre.ac.uk/id/eprint/45054

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