Begum, Asia and Gonzo, Martin ORCID: 0000-0002-9958-8785 (2023) Determining the effects of Moringa Oleifera on hepatic and monocytic cell lines in C1 Esterase inhibitor production. In: 13th C1-Inhibitor deficiency and Angioedema, 4th - 7th May 2023, Budapest.

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Abstract

Hereditary angioedema (HAE) is an autosomal dominant disorder, caused by a C1 esterase inhibitor (C1INH) deficiency or dysfunction. This rare disorder contributes to the reduced quality of life, considerable pain and debilitation and can be potentially fatal. Despite several therapeutic strategies for HAE, it remains a disease with substantial physical and economic burden for patients, with no actual cure and several adverse side effects posed from the current treatment options. The current treatments mainly focus on restoring the C1INH but not upregulating its production. Therefore, the main aim of this study was to maximise the release of C1INH by exposing the cells responsible for C1INH secretion to Moringa Oleifera in attempt to upregulate its production and subsequent secretion to the extracellular space.
Initial studies investigated the level of secreted C1INH by ELISA assay. HepG2 and THP-1 cells were stimulated by the addition to the culture medium of varying amounts of Moringa Oleifera. Culture supernatant samples were removed and the level of secreted C1INH was determined. Crude Moringa Oleifera seed was extracted using the solvent extraction method. Cell viability was determined for HepG2 and THP-1 cells before and after treatment using the trypan blue exclusion method and MTT assay. The amount and location of C1INH in HepG2 in the presence and absence of treatment was observed using florescence imaging. In this study, there was a significant difference observed after treatment with 1000mg/L of Moringa Oleifera on HepG2 cells. There was a significant difference shown for both HepG2 and THP-1 cells per day compared with the control irrespective of the concentrations. MTT data confirmed that the HepG2 has a hepatoprotective role after exposure to Moringa Oleifera. trypan blue exclusion method, showed a significant difference in cell viability for both cell lines after treatment with Moringa Olefiera compared with control. Overall, it can be concluded that HepG2 secretes a greater amount of C1INH and for the first time Moringa Oleifera showed the maximum C1INH secretion with maximum concentrations.

Item Type:	Conference or Conference Paper (Paper)
Uncontrolled Keywords:	Hereditary Angioedema; C1 Esterase inhibitor; Moringa Oleifera; hepatic cells; monocytic cells
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General) T Technology > T Technology (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Biomedical Science Research Group Faculty of Engineering & Science > School of Science (SCI)
Last Modified:	12 May 2023 10:39
URI:	http://gala.gre.ac.uk/id/eprint/42497

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