Covid-19; titration; neutralisation; storage; SARS-CoV-2; pseudotype; antibody

Di Genova, Cecilia, Sampson, Alex, Scott, Simon, Cantoni, Diego, Mayora-Neto, Martin, Bentley, Emma, Mattiuzzo, Giada, Wright, Edward, Derveni, Mariliza, Auld, Bethany, Harrison, Dale, Ferrara, Bill T. ORCID: 0000-0002-2163-4032 , Said, Mohamed, Selim, Arwa ORCID: 0000-0001-5552-7561 , Thompson, Elinor ORCID: 0000-0002-6434-9290 , Thompson, Craig, Carnell, George and Temperton, Nigel ORCID: 0000-0002-7978-3815 (2021) Production, titration, neutralisation and storage of SARS-CoV-2 lentiviral pseudotypes. Bio-protocol, 11 (21). pp. 1-21. ISSN 2331-8325 (doi:https://doi.org/10.21769/BioProtoc.4236)

DNA vaccines, cytomegalovirus, antibody, CTL, protection

Temperton, Nigel J. ORCID: 0000-0002-7978-3815 (2001) DNA vaccines against cytomegalovirus: current progress. International Journal of Antimicrobial Agents, 19 (3). pp. 169-172. ISSN 0924-8579 (doi:https://doi.org/10.1016/S0924-8579(01)00492-7)

H1N1 infection, immunological memory, cross-reactive immunity, avian H5N1 virus

Mahallawi, Waleed H., Kasbekar, Anand V., McCormick, Maxwell S., Hoschler, Katja, Temperton, Nigel ORCID: 0000-0002-7978-3815 , Leong, Samuel C., Beer, Helen, Ferrara, Francesca, McNamara, Paul S. and Zhang, Qibo (2013) Infection with 2009 H1N1 influenza virus primes for immunological memory in human nose-associated lymphoid tissue, offering cross-reactive immunity to H1N1 and avian H5N1 viruses. Journal of Virology, 87 (10). pp. 5331-5339. ISSN 0022-538X (Print), 1098-5514 (Online) (doi:https://doi.org/10.1128/JVI.03547-12)

H5N1, influenza, pseudotypes, serology, viral neutralization

Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Hoschler, Katja, Major, Diane, Nicolson, Carolyn, Manvell, Ruth, Hien, Vo Minh, Ha, Do Quang, de Jong, Menno, Zambon, Maria, Takeuchi, Yasuhiro and Weiss, Robin A. (2007) A sensitive retroviral pseudotype assay for influenza H5N1-neutralizing antibodies. Influenza and other respiratory viruses, 1 (3). pp. 105-112. ISSN 1750-2659 (online) (doi:https://doi.org/10.1111/j.1750-2659.2007.00016.x)

HPAI, H7 lentiviral pseudotype, LPAI, micro-neutralization, pandemic potential

Molesti, Eleonora, Cattoli, Giovanni, Ferrara, Francesca, Böttcher-Frieberthäuser, Eva, Terregino, Calogero and Temperton, Nigel ORCID: 0000-0002-7978-3815 (2013) The production and development of H7 Influenza virus pseudotypes for the study of humoral responses against avian viruses. Journal of Molecular and Genetic Medicine, 7. pp. 315-320. ISSN 1747-0862

Influenza, Neutralization, Pseudotypes, Serology, Standards, Validation

Ferrara, Francesca, Molesti, Eleonora, Scott, Simon, Cattoli, Giovanni and Temperton, Nigel ORCID: 0000-0002-7978-3815 (2017) The use of hyperimmune chicken reference sera is not appropriate for the validation of influenza pseudotype neutralization assays. Pathogens, 6 (4). ISSN 2076-0817 (Print), 2076-0817 (Online) (doi:https://doi.org/10.3390/pathogens6040045)

SARS, severe acute respiratory syndrome, CoV, coronaviru, PBMC, peripheral blood mononuclear cell, ICS, intracellular cytokine staining, SFC, spot forming cell, Nab, neutralizing Ab

Li, Chris Ka-fai, Wu, Hao, Yan, Huiping, Ma, Shiwu, Wang, Lili, Zhang, Mingxia, Tang, Xiaoping, Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Weiss, Robin A., Brenchley, Jason M., Douek, Daniel C., Mongkolsapaya, Juthathip, Tran, Bac-Hai, Lin, Chen-lung Steve, Screaton, Gavin R., Hou, Jin-lin, McMichael, Andrew J. and Xu, Xiao-Ning (2008) T Cell responses to whole SARS Coronavirus in humans. The Journal of Immunology, 181 (8). pp. 5490-5500. ISSN 0022-1767 (Print), 1550-6606 (Online) (doi:https://doi.org/10.4049/jimmunol.181.8.5490)

Trypanosoma cruzi, transfection, superoxide dismutase, overexpression

Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Wilkinson, Shane R., Meyer, David J. and Kelly, John M. (1998) Overexpression of superoxide dismutase in Trypanosoma cruzi results in increased sensitivity to the trypanocidal agents gentian violet and benznidazole. Molecular and Biochemical Parasitology, 96 (1-2). pp. 167-176. ISSN 0166-6851 (doi:https://doi.org/10.1016/S0166-6851(98)00127-3)

Trypanosoma cruzi; Iron superoxide dismutase; Oxidative stress

Temperton, Nigel ORCID: 0000-0002-7978-3815 , Wilkinson, Shane R. and Kelly, John M. (1996) Cloning of an Fe-superoxide dismutase gene homologue from Trypanosoma cruzi. Molecular and Biochemical Parasitology, 76 (1-2). pp. 339-343. ISSN 0166-6851 (doi:https://doi.org/10.1016/0166-6851(95)02553-7)

[1] These data were presented at the 3rd ESWI conference, Vilamoura, Portugal in September, 2008

Oha, Sawyin, Selleck, Paul, Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Chan, Paul K.S., Capecchi, Barbara, Manavis, Jim, Higgins, Geoff, Burrell, Christopher J. and Kok, Tuckweng (2009) Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses. Journal of Virological Methods, 57 (2). pp. 161-167. ISSN 0166-0934 (doi:https://doi.org/10.1016/j.jviromet.2008.12.016)

anti-bacterial agents biosynthesis, anti-bacterial agents chemistry, anti-bacterial agents pharmacology, drug design

Temperton, Nigel ORCID: 0000-0002-7978-3815 (2009) Society of general microbiology - 2009 spring meeting. Idrugs, 12 (5). pp. 294-296. ISSN 1369-7056 (Print), 2040-3410 (Online)

antibodies, immunization, influenza vaccine

Corti, Davide, Suguitan Jr., Amorsolo L., Pinna, Debora, Silacci, Chiara, Fernandez-Rodriguez, Blanca M., Vanzetta, Fabrizia, Santos, Celia, Luke, Catherine J., Torres-Velez, Fernando J., Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Weiss, Robin A., Sallusto, Federica, Subbarao, Kanta and Lanzavecchia, Antonio (2010) Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccine. The Journal of Clinical Investigation, 120 (5). pp. 1663-1673. ISSN 0021-9738 (Print), 1558-8238 (Online) (doi:https://doi.org/10.1172/JCI41902)

antigenic drift, H5N1, avian influenza virus, hemagglutinin, human influenza virus, mutation

Cattoli, Giovanni, Milani, Adelaide, Temperton, Nigel ORCID: 0000-0002-7978-3815 , Zecchin, Bianca, Buratin, Alessandra, Molesti, Eleonora, Meherez Aly, Mona, Arafa, Abdel and Capua, Ilaria (2011) Antigenic drift in H5N1 avian influenza virus in poultry is driven by mutations in major antigenic sites of the hemagglutinin molecule analogous to those for human influenza virus. Journal of Virology, 85 (17). pp. 8718-8724. ISSN 0022-538X (Print), 1098-5514 (Online) (doi:https://doi.org/10.1128/JVI.02403-10)

avian influenza, H5N1 virus, immunoprophylaxis, neutralizing mAbs, scFv

Ascione, Alessandro, Capecchi, Barbara, Campitelli, Laura, Imperiale, Valentina, Flegoa, Michela, Zamboni, Silvia, Gellini, Mara, Alberini, Isabella, Pittiglio, Eliana, Donatelli, Isabella, Temperton, Nigel J. ORCID: 0000-0002-7978-3815 and Cianfriglia, Maurizio (2009) Human monoclonal antibodies in single chain fragment variable format with potent neutralization activity against influenza virus H5N1. Antiviral Research, 83 (3). pp. 238-244. ISSN 0166-3542 (doi:https://doi.org/10.1016/j.antiviral.2009.05.005)

cytomegalovirus, DNA vaccine, CpG ODN, adjuvant

Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Quenelle, Debra C., Lawson, Keirissa M., Zuckerman, Jane M., Kern, Earl R., Griffiths, Paul D. and Emery, Vincent C. (2003) Enhancement of humoral immune responses to a human cytomegalovirus DNA vaccine: adjuvant effects of aluminum phosphate and CpG oligodeoxynucleotides. Journal of Medical Virology, 70 (1). pp. 86-90. ISSN 1096-9071 (on-line) (doi:https://doi.org/10.1002/jmv.10357)

disease outbreaks, prevention & control, birds, influenza, human

Temperton, N. ORCID: 0000-0002-7978-3815 (2009) BirdFlu2009: Avian Influenza and Human Health. 9-10 September 2009, Oxford, UK. IDrugs, 12 (11). pp. 686-688. ISSN 1369-7056 (Print), 2040-3410 (Online)

endogenous retrovirus, HERV, Sjogren’s syndrome, multiple sclerosis, autoimmune disease

Moyes, David L., Martin, Alex, Sawcer, Stephen, Temperton, Nigel ORCID: 0000-0002-7978-3815 , Worthington, Jane, Griffiths, David J. and Venables, Patrick J. (2005) The distribution of the endogenous retroviruses HERV-K113 and HERV-K115 in health and disease. Genomics, 86 (3). pp. 337-341. ISSN 0888-7543 (doi:https://doi.org/10.1016/j.ygeno.2005.06.004)

feline aminopeptidase N, type I feline coronaviruses, retroviral pseudotypes, cellular receptor

Dye, Charlotte, Temperton, Nigel ORCID: 0000-0002-7978-3815 and Siddell, Stuart G. (2007) Type I feline coronavirus spike glycoprotein fails to recognize aminopeptidase N as a functional receptor on feline cell lines. Journal of General Virology, 88 (6). pp. 1753-1760. ISSN 0022-1317 (Print), 1465-2099 (Online) (doi:https://doi.org/10.1099/vir.0.82666-0)

haemagglutinin, protease cleavage, antibody response, pseudotype serology

Ferrara, Francesca, Molesti, Eleonora, Böttcher-Frieberthäuser, Eva, Cattoli, Giovanni, Corti, Davide, Scott, Simon D. and Temperton, Nigel J. ORCID: 0000-0002-7978-3815 (2013) The human Transmembrane Protease Serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes. Journal of Molecular and Genetic Medicine, 7 (1):55. pp. 309-314. ISSN 1747-0862 (doi:https://doi.org/10.4172/1747-0862.1000055)

immunity, pseudotype assay, live SARS virus, neutralizing titers, infection, vaccines

Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Chan, Paul K., Simmons, Graham, Zambon, Maria C., Tedder, Richard S., Takeuchi, Yasuhiro and Weiss, Robin A. (2005) Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes. Emerging Infectious Diseases, 11 (3). pp. 411-416. ISSN 1080-6059 (doi:https://doi.org/10.3201/eid1103.040906)

influenza virus, nanobodies, H5N1

Ibanez, Lorena Itati, De Filette, Marina, Hultberg, Anna, Verrips, Theo, Temperton, Nigel ORCID: 0000-0002-7978-3815 , Weiss, Robin A., Vandevelde, Wesley, Schepens, Bert, Vanlandschoot, Peter and Saelens, Xavier (2011) Nanobodies with in vitro neutralizing activity protect mice against H5N1 influenza virus infection. The Journal of Infectious Diseases, 203 (8). pp. 1063-1072. ISSN 0022-1899 (Print), 1537-6613 (Online) (doi:https://doi.org/10.1093/infdis/jiq168)

lyssavirus, rabies virus, lagos bat virus, pseudotype, bat, Africa, Ghana, antibody, neutralisation, serology

Wright, Edward, Hayman, David T.S., Vaughan, Aisling, Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Wood, James L.N., Cunningham, Andrew A., Suu-Ire, Richard, Weiss, Robin A. and Fooks, Anthony R. (2010) Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses. Virology, 408 (2). pp. 183-189. ISSN 0042-6822 (doi:https://doi.org/10.1016/j.virol.2010.09.014)

microassay, immunity, rabies vaccines, lyssaviruses, rabies

Wright, Edward, Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Marston, Denise A., McElhinney, Lorraine M., Fooks, Anthiny R. and Weiss, Robin A. (2008) Investigating antibody neutralization of lyssaviruses using lentiviral pseudotypes: a cross-species comparison. Journal of General Virology, 89 (9). pp. 2204-2213. ISSN 0022-1317 (Print), 1465-2099 (Online) (doi:https://doi.org/10.1099/vir.0.2008/000349-0)

mutations, hemagglutinin, receptor binding, pH stability, PR8 influenza virus

Shelton, Holly, Roberts, Kim L., Molesti, Eleonora, Temperton, Nigel ORCID: 0000-0002-7978-3815 and Barclay, Wendy S. (2013) Mutations in hemagglutinin that affect receptor binding and pH stability increase replication of a PR8 influenza virus with H5 HA in the upper respiratory tract of ferrets and may contribute to transmissibility. Journal of General Virology, 94 (6). pp. 1220-1229. ISSN 0022-1317 (Print), 1465-2099 (Online) (doi:https://doi.org/10.1099/vir.0.050526-0)

neuraminidase inhibitor, pseudotype virus, antiviral, viral release assay, drug resistance,

Su, Ching-Yao, Wang, Shi-Yun, Shie, Jiun-Jie, Jeng, King-Song, Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Fang, Jim-Min, Wong, Chi-Huey and Cheng, Yih-Shyun E. (2008) In vitro evaluation of neuraminidase inhibitors using the neuraminidase-dependent release assay of hemagglutinin-pseudotyped viruses. Antiviral Research, 79 (3). pp. 199-205. ISSN 0166-3542 (doi:https://doi.org/10.1016/j.antiviral.2008.03.002)

neutralizing antibody, plasma cells, group 1, group 2, influenza A virus, hemagglutinins

Corti, Davide, Voss, Jarrod, Gamblin, Steven J., Codoni, Giosiana, Macagno, Annalisa, Jarrossay, David, Vachieri, Sebastien G., Pinna, Debora, Minola, Andrea, Vanzetta, Fabrizia, Silacci, Chiara, Fernandez-Rodriguez, Blanca M., Agatic, Gloria, Bianchi, Siro, Giacchetto-Sasselli, Isabella, Calder, Lesley, Sallusto, Federica, Collins, Patrick, Haire, Lesley F., Temperton, Nigel ORCID: 0000-0002-7978-3815 , Langedijk, Johannes P.M., Skehel, John J. and Lanzavecchia, Antonio (2011) A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins. Science, 333 (6044). pp. 850-856. ISSN 0036-8075 (Print), 1095-9203 (Online) (doi:https://doi.org/10.1126/science.1205669)

proteins, T. cruzi, peroxide-mediated damage, enzyme activity, cytoplasmic peroxiredoxin, mitochondrial peroxiredoxin

Wilkinson, Shane R., Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Mondragon, Angeles and Kelly, John M. (2000) Distinct mitochondrial and cytosolic enzymes mediate trypanothione-dependent peroxide metabolism in Trypanosoma cruzi. The Journal of Biological Chemistry, 275 (11). pp. 8220-8225. ISSN 0021-9258 (Print), 1083-351X (Online) (doi:https://doi.org/10.1074/jbc.275.11.8220)

pseudoparticle neutralization assay, avian influenza virus, H5N1, pandemic vaccine, cross-clade neutralization, serological methods

Alberini, Isabella, Del Tordello, Elena, Fasolo, Alba, Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Galli, Grazia, Gentile, Chiara, Montomoli, Emanuele, Hilbert, Anne K., Banzhoff, Angelika, Del Giudice, Giuseppe, Donnelly, John J., Rappuoli, Rino and Capecchi, Barbara (2009) Pseudoparticle neutralization is a reliable assay to measure immunity and cross-reactivity to H5N1 influenza viruses. Vaccine, 27 (43). pp. 5998-6003. ISSN 0264-410X (doi:https://doi.org/10.1016/j.vaccine.2009.07.079)

pseudotype virus, neutralizing antibody, H3N8, SRH, TMPRSS2

Scott, Simon, Molesti, Eleonora, Temperton, Nigel ORCID: 0000-0002-7978-3815 , Ferrara, Francesca, Böttcher-Friebertshäuser, Eva and Daly, Janet (2012) The use of equine influenza pseudotypes for serological screening. Journal of Molecular and Genetic Medicine, 6. pp. 304-308. ISSN 1747-0862

viral epitope, Llama, antibody fragments, respiratory syncytial virus, rabies virus, H5N1 Influenza

Hultberg, Anna, Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Rosseels, Valerie, Koenders, Mireille, Gonzalez-Pajuelo, Maria, Schepens, Bert, Ibanez, Lorena Itatı, Vanlandschoot, Peter, Schillemans, Joris, Saunders, Michael, Weiss, Robin A., Saelens, Xavier, Melero, Jose A., Verrips, C. Theo, Van Gucht, Steven and de Haard, Hans J. (2011) Llama-derived single domain antibodies to build multivalent, superpotent and broadened neutralizing anti-viral molecules. PLoS ONE, 6 (4). pp. 1-12. ISSN 1932-6203 (on-line) (doi:https://doi.org/10.1371/journal.pone.0017665)

virus infection, gene-to-vaccine, influenza vaccination

Loureiro, Silvia, Ren, Junyuan, Phapugrangkul, Pongsathon, Colaco, Camilo A., Bailey, Christopher R., Shelton, Holly, Molesti, Eleonora, Temperton, Nigel J. ORCID: 0000-0002-7978-3815 , Barclay, Wendy S. and Jones, Ian M. (2010) Adjuvant-free immunization with hemagglutinin-Fc fusion proteins as an approach to influenza vaccines. Journal of Virology, 85 (6). pp. 3010-3014. ISSN 0022-538X (Print), 1098-5514 (Online) (doi:https://doi.org/10.1128/​JVI.01241-10)