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The use of hyperimmune chicken reference sera is not appropriate for the validation of influenza pseudotype neutralization assays

The use of hyperimmune chicken reference sera is not appropriate for the validation of influenza pseudotype neutralization assays

Ferrara, Francesca, Molesti, Eleonora, Scott, Simon, Cattoli, Giovanni and Temperton, Nigel ORCID: 0000-0002-7978-3815 (2017) The use of hyperimmune chicken reference sera is not appropriate for the validation of influenza pseudotype neutralization assays. Pathogens, 6 (4). ISSN 2076-0817 (Print), 2076-0817 (Online) (doi:https://doi.org/10.3390/pathogens6040045)

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Abstract

The pseudotype particle neutralization test (pp-NT) is a next-generation serological assay employed for the sensitive study of influenza antibody responses against hemagglutinin (HA), including stalk-directed antibodies. However, a validation of this assay has yet to be performed, and this limits its use to primarily research laboratories. To identify possible serological standards to be used in optimization and validation of the pp-NT, we have evaluated the cross-reactivity of hyperimmune chicken reference antisera in this assay. Our findings show that the cross-reactivity detected by the pp-NT is only partly explained by phylogenetic relationships and protein homology between the HA subtypes analysed; further studies are necessary to understand the origin of the cross-reactivity detected, and reference standards with higher specificity should be evaluated or generated de novo for future use in pp-NT.

Item Type: Article
Additional Information: © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Uncontrolled Keywords: Influenza, Neutralization, Pseudotypes, Serology, Standards, Validation
Faculty / School / Research Centre / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Medway School of Pharmacy
SWORD Depositor: Publications Router
Last Modified: 20 Nov 2017 12:52
URI: http://gala.gre.ac.uk/id/eprint/17769

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