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The production and development of H7 Influenza virus pseudotypes for the study of humoral responses against avian viruses

The production and development of H7 Influenza virus pseudotypes for the study of humoral responses against avian viruses

Molesti, Eleonora, Cattoli, Giovanni, Ferrara, Francesca, Böttcher-Frieberthäuser, Eva, Terregino, Calogero and Temperton, Nigel ORCID: 0000-0002-7978-3815 (2013) The production and development of H7 Influenza virus pseudotypes for the study of humoral responses against avian viruses. Journal of Molecular and Genetic Medicine, 7. pp. 315-320. ISSN 1747-0862

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Abstract

In recent years, high pathogenicity avian influenza (HPAI) virus, H5N1, low pathogenicity avian influenza (LPAI) virus, H9N2, and both HPAI and LPAI H7 viruses have proved devastating for the affected economies reliant on poultry industry, and have posed serious public health concerns. These viruses have repeatedly caused zoonotic disease in humans, raising concerns of a potential influenza pandemic. Despite the focus on the HPAI H5N1 outbreak in 1997 some H7 strains have also shown to be occasionally adaptable to infecting humans. Therefore, applying knowledge of the H5 virus evolution and spread to the development of sensitive
serological methods is likely to improve our ability to understand and respond to the emergence of other HPAI and LPAI viruses, present within the avian populations, with the potential to infect humans and other species. In the present study we describe the construction and production of lentiviral pseudotypes bearing envelope glycoproteins of LPAI and HPAI H7 avian influenza viruses, which have been responsible for several outbreaks in the past decade. The H7 pseudotypes were evaluated in pseudotype-based neutralization (pp-NT) assays in order to detect and quantify the presence of neutralizing antibodies in avian sera, which were confirmed H7 positive by inhibition of haemagglutination (HI) test. Overall, our results substantiate influenza virus pseudotype neutralization as a robust tool for influenza sero-surveillance.

Item Type: Article
Additional Information: [1] Published online: 19 February 2013. [2] Citation: Molesti, Eleonora, Cattoli, Giovanni, Ferrara, Francesca, Böttcher-Frieberthäuser, Eva, Terregino , Calogero and Temperton, Nigel (2013) The production and development of H7 Influenza virus pseudotypes for the study of humoral responses against avian viruses. Journal of Molecular and Genetic Medicine, 7. pp. 315-320. ISSN 1747-0862. [3] Copyright: The Author(s). First Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.
Uncontrolled Keywords: HPAI, H7 lentiviral pseudotype, LPAI, micro-neutralization, pandemic potential
Subjects: R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
R Medicine > RS Pharmacy and materia medica
Faculty / Department / Research Group: Faculty of Engineering & Science > Medway School of Pharmacy
Related URLs:
Last Modified: 28 Apr 2016 03:38
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
URI: http://gala.gre.ac.uk/id/eprint/9574

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