Richter, Loandi, Duvenage, Stacey ORCID: https://orcid.org/0000-0002-5984-1491, Du Plessis, Erika Margaret, Msimango, Thabang, Dlangalala, Manana, Mathavha, Muneiwa Tshidino, Molelekoa,, Tintswalo, Kgoale, Degracious and Korsten, Lise (2024) Genomic evaluation of multidrug-resistant extended-spectrum β-lactamase (ESBL)-producing Escherichia coli from irrigation water and fresh produce in South Africa: a cross-sectional analysis. Environmental Science and Technology, 58 (32). pp. 14421-14438. ISSN 0013-936X (Print), 1520-5851 (Online) (doi:https://doi.org/10.1021/)

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Abstract

Escherichia coli, both commensal and pathogenic, can colonize plants and persist in various environments. It indicates fecal contamination in water and food and serves as a marker of antimicrobial resistance. In this context, 61 extended-spectrum β-lactamase (ESBL)-producing E. coli from irrigation water and fresh produce from previous studies were characterized using whole genome sequencing (Illumina MiSeq). The Center for Genomic Epidemiology and Galaxy platforms were used to determine antimicrobial resistance genes, virulence genes, plasmid typing, mobile genetic elements, multilocus sequence typing (MLST), and pathogenicity prediction. In total, 19 known MLST groups were detected among the 61 isolates. Phylogroup B1 (ST58) and Phylogroup E (ST9583) were the most common sequence types. The six ST10 (serotype O101:H9) isolates carried the most resistance genes, spanning eight antibiotic classes. Overall, 95.1% of the isolates carried resistance genes from three or more classes. The blaCTX-M-1, blaCTX-M-14,

Item Type:	Article
Uncontrolled Keywords:	One Health, antimicrobial resistance, AMR, whole genome sequencing, WGS, food safety, environmental surveillance, ExPEC
Subjects:	Q Science > Q Science (General) Q Science > QR Microbiology Q Science > QR Microbiology > QR355 Virology
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Centre for Food Systems Research Faculty of Engineering & Science > Natural Resources Institute > Centre for Food Systems Research > Food Safety and Quality
Last Modified:	22 Nov 2024 11:11
URI:	http://gala.gre.ac.uk/id/eprint/47539

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