Lv, Xue, Zhang, Xinyang, Wuyun, Tana, Yang, Huiyi, Yu, Shuhan and Zhang, Lu (2025) A comparative analysis of physiological, biochemical, and molecular responses to elevated ozone identifies specific mechanisms of Kentucky bluegrass resistant cultivar. Journal of Environmental Sciences (JES). ISSN 1001-0742 (Print), 1878-7320 (Online) (In Press)

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Abstract

Rapid urbanization and industrialization have heightened near-surface concentrations of ozone (O3), causing oxidative damage in plants like turfgrass. Most studies on turfgrass responses to O3 focus primarily on physiological aspects, with few exploring the underlying molecular mechanisms. To fill this gap, we analyzed the phenotypic, physiological, transcriptomic, and metabolomic differences of two Kentucky bluegrass (Poa pratensis L.) cultivars (‘Arcadia’ and ‘Action’) under elevated O3 (EO3, non-filtered ambient air mixed with 80 ppb O3) and non-filtered ambient air (NF). After 9 days, the sensitive cultivar ‘Action’ showed stronger ozone-related responses than the resistant cultivar ‘Arcadia’, including leaf visible injury, reduced chlorophyll content, lower photosynthetic rate, and increased H2O2 and MDA content. Microscopic analysis of epidermal cell structure revealed that ‘Action’ had higher stomatal density than ‘Arcadia’, potentially resulting in higher O3 influx. The functional enrichment analysis showed that the differentially expressed genes (DEGs) between EO3 and NF in both cultivars were mainly related to signal transduction, redox control, and metabolism. The up-regulation of genes related to the JA signaling pathway (including OPR and DOX) in the resistant cultivar may boost its O3 resistance. In contrast, the sensitive cultivar activated the transcription factor WRKY33 via MAPK signaling, promoting the up-regulation of glutathione metabolism genes to maintain redox balance. In addition, the metabolomic analysis showed that ‘Arcadia’ had higher flavonoid content under O3 stress than ‘Action’, likely helping it maintain redox homeostasis.

Item Type:	Article
Uncontrolled Keywords:	antioxidant defense, flavonoids, metabolome, ozone stress, Poa pratensis, transcriptome
Subjects:	Q Science > Q Science (General) S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Centre for Society, Environment and Development (CSED) Faculty of Engineering & Science > Natural Resources Institute > Centre for Society, Environment and Development (CSED) > Climate Change
Last Modified:	22 May 2025 11:08
URI:	http://gala.gre.ac.uk/id/eprint/50511

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