Silva, Goncalo ORCID: https://orcid.org/0000-0001-5544-2947 and Boonham, Neil (2025) Detection of cassava mosaic geminiviruses using Loop-Mediated Isothermal Amplification (LAMP). In: Zerbini, F.M., Fiallo-Olivé, E. and Navas-Castillo, J., (eds.) Geminiviruses: Methods in Molecular Biology. Methods in Molecular Biology ((MIMB), 2912 . Humana - Springer Protocols, Springer Nature, New York, NY, pp. 157-164. ISBN 978-1071644539; 978-1071644546 (doi:10.1007/978-1-0716-4454-6_13)

PDF (Accepted Book Chapter) 50082 SILVA_Detection_Of_Cassava_Mosaic_Geminiviruses_Using_Loop-Mediated_Isothermal_Amplification (LAMP)_(AAM BOOK CHAPTER)_2025.pdf - Accepted Version Restricted to Repository staff only until 13 March 2026. Download (304kB) | Request a copy

Abstract

A real-time loop-mediated isothermal amplification (LAMP) is described to diagnose cassava mosaic disease (CMD). The disease continues to cause great damage in Sub-Saharan African countries threatening food security. Early detection of the pathogen is paramount to control the spread of the disease and promote the safe exchange of clean planting material. Real-time LAMP in combination with a crude but rapid method of DNA extraction can be used to detect the two most widely distributed species of cassava mosaic geminiviruses in Sub-Saharan Africa, African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV), in less than 30 min at a single operation temperature (65 °C) without the need for any expensive instrumentation. Further, this protocol avoids the laborious extraction of high-quality DNA and making it suitable to be used in poorly resourced laboratories and in field settings.

Item Type:	Book Section
Additional Information:	Accepted Manuscripts (AMs) of non-OA content are subject to Springer Nature’s AM Terms of Use. - MP
Uncontrolled Keywords:	Begomovirus, ACMV, EACMV, LAMP, on-site detection, virus diagnostics
Subjects:	Q Science > Q Science (General) Q Science > QR Microbiology Q Science > QR Microbiology > QR355 Virology
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health > Molecular Virology & Entomology
Last Modified:	19 Mar 2025 15:18
URI:	http://gala.gre.ac.uk/id/eprint/50082

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