Ranatunga, Medhavi, Deacon, Andrew D. ORCID: 0000-0001-7630-4973 , Harbige, Laurence S., Dyer, Paul, Boateng, Joshua ORCID: 0000-0002-6310-729X and Getti, Giulia ORCID: 0000-0003-1402-8496 (2024) Ex vivo analysis of the association of GFP-expressing L. aethiopica and L. mexicana with human Peripheral Blood-Derived (PBD) leukocytes over 24 Hours. Microorganisms, 12 (9):1909. ISSN 2076-2607 (Online) (doi:https://doi.org/10.3390/microorganisms12091909)

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PDF (VoR (OA)) 48342 RANATUNGA_Ex_Vivo_Analysis_of_the_Association_of_GFP-Expressing_L_aethiopica_and_L_mexicana_with_Human_Peripheral_Blood-Derived_(PBD)_Leukocytes_over_24_Hours_(OA)_2024.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview

Abstract

Leishmania parasites are transmitted to mammalian hosts through the bite of sandflies. These parasites can infect phagocytic cells (macrophages, dendritic cells, and neutrophils) and non-phagocytic cells (B cells and fibroblasts). In mice models, the disease development or resolution is linked to T cell responses involving inflammatory cytokines and the activation of macrophages with the M1/M2 phenotype. However, this mechanism does not apply to human infection where a more complex immunological response occurs. The understanding of interactions between immune cells during Leishmania infection in humans is still limited, as current infection models focus on individual cell types or late infection using controlled human infection models (CHIMs). This study investigated the early parasite infection in freshly isolated peripheral blood-derived (PBD) leukocytes over 24 h. Flow cytometer analysis is used in immunophenotyping to identify different subpopulations. The study found that among the L. aethiopicaGFP-asso

Item Type:	Article
Additional Information:	This article belongs to the Special Issue Research on Leishmania and Leishmaniasis.
Uncontrolled Keywords:	peripheral blood-derived leukocytes, ex vivo, L. aethiopicaGFP, L. mexicanaGFP, CD4+, naïve, activated, effector, regulatory T cells
Subjects:	Q Science > Q Science (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > School of Science (SCI)
Last Modified:	18 Oct 2024 09:40
URI:	http://gala.gre.ac.uk/id/eprint/48342

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