Naganur, Priya, Shankarappa, Kodegandlu Subbanna, Mesta, Raghavendra K., Rao, Chilakalapudi Durga, Venkataravanappa, Venkataravanappa, Maruthi, Midatharahally Narasegowda ORCID: 0000-0002-8060-866X and Narasimha Reddy, Lakshminarayana Reddy C. (2023) Detecting tomato leaf curl New Delhi virus causing ridge gourd yellow mosaic disease, and other Begomoviruses by antibody-based methods. Plants, 12:490. ISSN 2223-7747 (Online) (doi:https://doi.org/10.3390/plants12030490)

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Abstract

The incidence and severity of begomovirus diseases have been increasing around the world recently, and the ridge gourd [Luffa acutangula (Roxb.) L.] is the latest example of a crop that
has become highly susceptible to the outbreak of the tomato leaf curl New Delhi virus (ToLCNDV, genus Begomovirus) in India. Accurate diagnosis of causal agents is important in designing disease management strategies. In this study the coat protein (CP) gene from a ToLCNDV-Rgridge gourd isolate was used to produce polyclonal antibodies (ToLCNDV-Rg-CP-PAb) in a rabbit. The antibodies successfully detected a 30.5 kDa ToLCNDV-Rg-CP in extracts of symptomatic ridge gourd leaf samples by several assays, such as Western Blotting (WB), Dot Immuno Binding Assay (DIBA), Direct Antigen Coating Enzyme Linked Immuno Sorbent Assay (DAC-ELISA), Immuno Capture Polymerase Chain Reaction (IC-PCR), and Immuno Capture Loop-Mediated Isothermal Amplification (IC-LAMP) assays. However, none of the negative samples tested positive in either of the detection methods. Among all the methods tested, the immunocapture assay, IC-LAMP, was the most sensitive in detecting ToLCNDV-Rg. Furthermore, antibodies generated in this study also detected other commonly occurring begomoviruses in South India, such as tomato leaf curl Palampur virus and squash leaf curl China virus in cucurbits. Together, ToLCNDV-Rg-CP-PAb can be used for detecting at least three species of begomoviruses infecting cucurbits. The obtained antibodies will contribute to monitoring disease outbreaks in multiple crops.

Item Type:	Article
Uncontrolled Keywords:	begomovirus, coat protein, ELISA, Geminiviridae, immunocapture assays, ridge gourd, tomato leaf curl New Delhi virus
Subjects:	Q Science > Q Science (General) Q Science > QR Microbiology S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health > Plant Disease & Vectors
Last Modified:	07 Oct 2024 13:10
URI:	http://gala.gre.ac.uk/id/eprint/48254

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