Protocol for rearing and using mosquitoes for flight path tracking and behavioral characterization in wind tunnel bioassays
Tools
Tools
Carnaghi, Manuela
ORCID: 0000-0003-4595-082X
, Mandelli, Federico, Feugère, Lionel
ORCID: 0000-0003-0883-5224
, Joiner, Jillian, Belmain, Steven R.
ORCID: 0000-0002-5590-7545
, Hopkins, Richard J.
ORCID: 0000-0003-4935-5825
and Hawkes, Frances M.
ORCID: 0000-0002-0964-3702
(2024)
Protocol for rearing and using mosquitoes for flight path tracking and behavioral characterization in wind tunnel bioassays.
STAR Protocols, 5 (3):103180.
ISSN 2666-1667 (Online)
(doi:https://doi.org/10.1016/j.xpro.2024.103180)
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PDF (Open Access Article)
47598_HAWKES_Protocol_Mosquito_Flight_(OA)_2024.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (4MB) | Preview |
Abstract
Mosquito behavioral assays are an important component in vector research and control tool development. Here, we present a protocol for rearing Anopheles mosquitoes, performing host-seeking behavioral bioassays, and collecting 3D flight tracks in a large wind tunnel.We describe steps for setting up host-seeking landing assays, both as a non-choice and as a dual-choice assay, and analyzing flight tracks. This protocol can be applied in the research of several behavioral traits, including nectar seeking, resting, mating, and oviposition behavior. For complete details on the use and execution of this protocol, please refer to Carnaghi et al.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | workflow instructions, Anopheles, host-seeking behavioral studies |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Faculty / School / Research Centre / Research Group: | Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health > Behavioural Ecology |
| Last Modified: | 02 Sep 2024 15:34 |
| URI: | http://gala.gre.ac.uk/id/eprint/47598 |
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