Carnaghi, Manuela ORCID: https://orcid.org/0000-0003-4595-082X, Mandelli, Federico, Feugère, Lionel ORCID: https://orcid.org/0000-0003-0883-5224, Joiner, Jillian, Belmain, Steven R. ORCID: https://orcid.org/0000-0002-5590-7545, Hopkins, Richard J. ORCID: https://orcid.org/0000-0003-4935-5825 and Hawkes, Frances M. ORCID: https://orcid.org/0000-0002-0964-3702 (2024) Protocol for rearing and using mosquitoes for flight path tracking and behavioral characterization in wind tunnel bioassays. STAR Protocols, 5 (3):103180. ISSN 2666-1667 (Online) (doi:10.1016/j.xpro.2024.103180)

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Abstract

Mosquito behavioral assays are an important component in vector research and control tool development. Here, we present a protocol for rearing Anopheles mosquitoes, performing host-seeking behavioral bioassays, and collecting 3D flight tracks in a large wind tunnel.We describe steps for setting up host-seeking landing assays, both as a non-choice and as a dual-choice assay, and analyzing flight tracks. This protocol can be applied in the research of several behavioral traits, including nectar seeking, resting, mating, and oviposition behavior. For complete details on the use and execution of this protocol, please refer to Carnaghi et al.

Item Type:	Article
Uncontrolled Keywords:	workflow instructions, Anopheles, host-seeking behavioral studies
Subjects:	Q Science > QH Natural history > QH301 Biology
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health > Behavioural Ecology
Last Modified:	02 Sep 2024 15:34
URI:	http://gala.gre.ac.uk/id/eprint/47598

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