Tighsazzadeh, Mohammad and Boateng, Joshua ORCID: https://orcid.org/0000-0002-6310-729X (2024) Matrix hyaluronic acid and bilayer poly-hydroxyethyl methacrylate-hyaluronic acid films as potential ocular drug delivery platforms. International Journal of Biological Macromolecules, 260 (Part 2):129496. ISSN 0141-8130 (doi:10.1016/j.ijbiomac.2024.129496)

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Abstract

This study aimed to design hydrogel-based films comprising hyaluronic acid (HA) to overcome limitations of currently used eye drops. Timolol-loaded crosslinked (X2) HA-based and bilayer (HA/pHEMA-based layers) (B2) films were designed and characterized. The films were transparent (UV, visual observation) with crosslinked (<80%) films showing lower light transmittance than bilayer (> 80%) films. X2 showed significantly higher swelling capacity, tensile strength and elastic modulus (5491.6%, 1539.8Nmm-2, 1777.2mPa) than B2 (1905.0%, 170.0Nmm-2, 67.3mPa) respectively. However, X2 showed lower cumulative drug released, adhesive force (27.3%, 6.2N) than B2 (57.5%, 8.6N). UV sterilization did not significantly alter physical properties, while SEM/IR microscopy showed smooth surface morphology and homogeneous drug distribution. Timolol permeation (EpiCornealTM/porcine cornea) depended on the film matrix with erodible films showing similar permeation to commercial eyedrops. Drug permeation for porcine cornea (X2=549.0.2, B2=312.1 µgcm-2h-1) was significantly faster than EpiCornealTM (X2=55.2, B2=37.6 µgcm-2h-1), but with a linear correlation between them. All the selected optimized films showed acceptable compatibility (MTT assay) with both HeLa cells and EpiCornealTM. In conclusion, crosslinked and bilayer HA based films showed ideal characteristics suitable for potential ocular drug delivery, though further work is required to further optimize these properties and confirm their efficacy including in vivo tests.

Item Type:	Article
Uncontrolled Keywords:	EpiCorneal™; glaucoma; hyaluronic acid; porcine cornea; poly-hydroxyethyl methacrylate; Timolol
Subjects:	Q Science > Q Science (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > School of Science (SCI)
Last Modified:	06 Feb 2024 12:53
URI:	http://gala.gre.ac.uk/id/eprint/45329

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