Tumbapo, Sobha, Strudwick, Adam, Stastna, Jana, Harvey, Simon ORCID: https://orcid.org/0000-0002-7504-2227 and Bloemink, Marieke (2023) Moderate dietary restriction delays the onset of age-associated sarcopenia in Caenorhabditis elegans due to reduced myosin UNC-54 degradation. Mechanisms of Ageing and Development, 217:111900. ISSN 0047-6374 (doi:10.1016/j.mad.2023.111900)

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Abstract

Sarcopenia, a gradual decrease in skeletal muscle mass and strength, is a major component of frailty in the elderly, with age, (lack of) exercise and diet found to be the major risk factors. The nematode Caenorhabditis elegans is an important model of sarcopenia. Although many studies describe loss of muscle function in ageing C. elegans, surprisingly few report on the loss of muscle mass. Here, in order to quantify loss of muscle mass under various dietary restriction (DR) conditions, we used an internal GFP standard to determine levels of the major body wall muscle myosin (UNC-54) in transgenic unc-54::gfp worms over their lifespan. Myosin density linearly increased during the first week of adulthood and there was no significant effect of DR. In contrast, an exponential decrease in myosin density was seen during the second week of adulthood, with reduced rates of myosin loss for mild and medium DR compared to control. UNC-54 turnover rates, previously determined using pulse-labelling methods, correspond well with the t1/2 value found here for UNC-54-GFP using fluorescence (control t1/2 = 12.0 days), independently validating our approach. These data indicate that sarcopenia is delayed in worms under mild and medium DR due to a reduced rate of myosin UNC-54 degradation, thereby maintaining protein homeostasis.

Item Type:	Article
Uncontrolled Keywords:	aging; sarcopenia; C. elegans; healthspan; myosin UNC-54
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Education, Health & Human Sciences Faculty of Education, Health & Human Sciences > Institute for Lifecourse Development
Last Modified:	16 May 2024 12:03
URI:	http://gala.gre.ac.uk/id/eprint/45309

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