Dey, Tushar K. ORCID: https://orcid.org/0000-0002-9184-449X, Shome, Bibek R., Bandyopadhyay, Samiran, Goyal, Naresh Kumar, Lundkvist, Åke ORCID: https://orcid.org/0000-0001-8608-6551, Deka, Ram P. ORCID: https://orcid.org/0000-0002-8120-224X, Shome, Rajeswari, Venugopal, Nimita, Grace, Delia ORCID: https://orcid.org/0000-0002-0195-9489, Sharma, Garima, Rahman, Habibar and Lindahl, Johanna F. ORCID: https://orcid.org/0000-0002-1175-0398 (2023) Molecular Characterization of Methicillin-Resistant Staphylococci from the Dairy Value Chain in Two Indian States. Pathogens, 12 (2). p. 344. ISSN 2076-0817 (doi:10.3390/pathogens12020344)

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Abstract

Bovine milk and milk products may contain pathogens, antimicrobial resistant bacteria, and antibiotic residues that could harm consumers. We analyzed 282 gram-positive isolates from milk samples from dairy farmers and vendors in Haryana and Assam, India, to assess the prevalence of methicillin-resistant staphylococci using microbiological tests, antibiotic susceptibility testing, and genotyping by PCR. The prevalence of genotypic methicillin resistance in isolates from raw milk samples was 5% [95% confidence interval, CI (3–8)], with 7% [CI (3–10)] in Haryana, in contrast to 2% [CI (0.2–6)] in Assam. The prevalence was the same in isolates from milk samples collected from farmers [5% (n = 6), CI (2–11)] and vendors [5% (n = 7), CI (2–10)]. Methicillin resistance was also observed in 15% of the isolates from pasteurized milk [(n = 3), CI (3–38)]. Two staphylococci harboring a novel mecC gene were identified for the first time in Indian dairy products. The only SCCmec type identified was Type V. The staphylococci with the mecA (n = 11) gene in raw milk were commonly resistant to oxacillin [92%, CI (59–100)] and cefoxitin [74%, CI (39–94)], while the isolates with mecC (n = 2) were resistant to oxacillin (100%) only. All the staphylococci with the mecA (n = 3) gene in pasteurized milk were resistant to both oxacillin and cefoxitin. Our results provided evidence that methicillin-resistant staphylococci occur in dairy products in India with potential public health implications. The state with more intensive dairy systems (Haryana) had higher levels of methicillin-resistant bacteria in milk.

Item Type:	Article
Uncontrolled Keywords:	AMR, LMIC
Subjects:	Q Science > Q Science (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Centre for Food Systems Research Faculty of Engineering & Science > Natural Resources Institute > Centre for Food Systems Research > Food Safety and Quality
Last Modified:	27 Nov 2024 14:49
URI:	http://gala.gre.ac.uk/id/eprint/41541

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