Li, He ORCID: https://orcid.org/0000-0001-9345-042X, Wang, Rongchun, Wang, Lifeng, Li, Lin, Ma, Ying and Zhou, Shaobo ORCID: https://orcid.org/0000-0001-5214-2973 (2020) Bovine milk fat globule epidermal growth factor VIII activates PI3K/Akt signaling pathway and attenuates sarcopenia in rat model induced by d-galactose. Food Bioscience, 40:100847. ISSN 2212-4292 (Print), 2212-4306 (Online) (doi:10.1016/j.fbio.2020.100847)

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Abstract

To develop a more effective and safer treatment for sarcopenia, this research investigated the anti-sarcopenia mechanism of Milk Fat Globule Epidermal Growth Factor Ⅷ (MFG-E8) from the liver function and metabolism in sarcopenic model rat. After 4 weeks nutritional intervention experiment, MFG-E8 can significantly increase the gastrocnemius mass in rat. The mechanism of MFG-E8 in improving sarcopenia was related to its promotional capacity to the activities of superoxide dismutase (SOD) activity in serum, Glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) in liver. Meanwhile, MFG-E8 could also down-regulate obesity-related indicators, such as triglyceride (TG) and Non-esterified fatty acid (NEFA). The analysis of liver and gastrocnemius histopathology found that MFG-E8 could reduce the accumulation of fatty vesicles, improve liver function, thereby alleviating gastrocnemius tissue inflammation. In vitro experiments, myoblasts obtained from gastrocnemius tissue showed that MFG-E8 could reduce mitochondrial autophagy and inhibit cell apoptosis. In addition, MFG-E8 could up-regulate the phosphorylation level of PI3K via activating PI3K/Akt signaling pathway in gastrocnemius tissue, and promote the formation of muscle fibers, thereby increasing muscle mass. Moreover, MFG-E8 could also promote the formation of neuromuscular junctions by up-regulating the mRNA and protein expression of MusK in gastrocnemius.

Item Type:	Article
Uncontrolled Keywords:	MFG-E8Sarcopenic model ratMyoblastsPI3K/Akt signaling pathway
Subjects:	Q Science > Q Science (General) R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine R Medicine > RM Therapeutics. Pharmacology
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > School of Science (SCI)
Last Modified:	24 Mar 2023 11:28
URI:	http://gala.gre.ac.uk/id/eprint/39032

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