Pang, Wei, Lu, Shuqing, Zheng, Rong, Li, Xin, Yang, Shunbo, Feng, Yuxia, Wang, Shuqiu ORCID: https://orcid.org/0000-0003-1069-6606, Guo, Jin ORCID: https://orcid.org/0000-0002-6149-9875 and Zhou, Shaobo ORCID: https://orcid.org/0000-0001-5214-2973 (2022) Investigation into antiepileptic effect of ganoderic acid A and its mechanism in seizure rats induced by pentylenetetrazole. BioMed Research International, 2022:5940372. pp. 1-14. ISSN 2314-6133 (Print), 2314-6141 (Online) (doi:10.1155/2022/5940372)

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Abstract

Ganoderic acid A (GAA) exhibited neuron protection in in vitro epilepsy study, but no study has been done in vivo. Rats were
administered (i.p.) pentylenetetrazole daily for 28 days to induce seizure. Rats with grade II or above of epileptic score were
divided into three groups and given placebo, sodium valproate, or GAA treatment, respectively, for 7 days. The electrical
signals of brain were monitored with electroencephalography (EGG); epileptic behavior was assessed using the Racine scale;
morphological changes and apoptosis rate of cortical neurons were assessed with H&E staining and TUNEL staining,
respectively. Protein expression of calcium-sensing receptor, p-ERK, p-JNK, and p-p38 in hippocampal tissue and Bcl-2,
cleaved caspase-3, and Bax in cortical tissues was observed by Western blot and immunohistochemistry assay, respectively.
After GAA treatment, apparent seizure-like EEG with significant arrhythmic disorder and spike waves was reduced or
disappeared, and wave amplitude of EEG was reduced significantly. GAA showed similar effect with sodium valproate
treatments on epilepsy. There were an apparent improvement of the epileptic behavior and a significant increase in the
epileptic latency and shortening of the epileptic duration in the treatment group compared to control. GAA treatment
ameliorated the nuclear pyknosis of neurons which appeared seriously in the epilepsy group. GAA treatment significantly
reduced the cortical neuron apoptosis of epilepsy and the expression of calcium-sensing receptor, p-P38, p-JNK, cleaved
caspase-3, and Bax but increased the expression of both p-ERK and Bcl-2. In conclusion, GAA treatment showed strong
antiepileptic effect by decreasing apoptosis in cortical neuron and the expression of calcium-sensing receptor and stimulating
the MAPK pathway

Item Type:	Article
Uncontrolled Keywords:	antiepileptic effect; ganoderic acid A; seizure rats; pentylenetetrazole
Subjects:	Q Science > Q Science (General) R Medicine > RS Pharmacy and materia medica
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > School of Science (SCI)
Last Modified:	20 Dec 2022 16:41
URI:	http://gala.gre.ac.uk/id/eprint/38348

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