Kavil, Siji, Otti, Gerald, Armitage, Andrew ORCID: https://orcid.org/0000-0002-0610-763X, Bouvaine, Sophie ORCID: https://orcid.org/0000-0002-0788-3243 and Maruthi, M.N. ORCID: https://orcid.org/0000-0002-8060-866X (2021) PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava. Virology Journal, 18:184. ISSN 1743-422X (Print), 1743-422X (Online) (doi:10.1186/s12985-021-01649-2)

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Abstract

Background: The phenylalanine ammonia lyase genes play crucial role in plant response to biotic and abiotic stresses. In this study, we characterized the role of PAL genes in increasing resistance to the Cassava brown streak virus that causes the economically important cassava brown streak disease (CBSD) on cassava in Africa. Methods: The whole transcriptomes of eight cassava varieties differing in resistance to CBSD were obtained at 1, 5 and 8 weeks after CBSV infection. Results: Analysis of RNA-Seq data identified the overexpression of PAL1, PAL2, cinnamic acid and two chalcone synthase genes in CBSD-resistant cassava varieties, which was subsequently confirmed by RT-qPCR. The exogenous application of Acibenzolar-S-Methyl induced PAL1 gene expression to enhance resistance in the susceptible var. Kalawe. In contrast, the silencing of PAL1 by RNA interference led to increased susceptibility of the resistant var. Kaleso to CBSD. Conclusions: PAL1 gene of the phenylpropanoid pathway has a major role in inducing resistance to CBSD in cassava plants and its early induction is key for CBSD resistance.

Item Type:	Article
Uncontrolled Keywords:	Cassava, CBSD, PAL1, resistance, induction
Subjects:	S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health > Molecular Virology & Entomology
Related URLs:	Publisher http://pubmed.ncbi.nlm.nih.gov/34503522/
Last Modified:	27 Nov 2024 15:18
URI:	http://gala.gre.ac.uk/id/eprint/33981

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