Harrison, Dale J. A. and Thompson, Elinor P ORCID: 0000-0002-6434-9290 (2020) A rapid and low-cost method for genomic DNA extraction from the cyanobacterium Synechocystis. Biology Methods and Protocols, 5 (1):bpaa011. ISSN 2396-8923 (Online) (doi:https://doi.org/10.1093/biomethods/bpaa011)

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Abstract

A two-step method is reported for preparation of genomic DNA from the model cyanobacterium Synechocystis that can be performed with minimal equipment and reagents in about an hour. High yields of genetic material can be obtained (200–450 ng/μl) with reasonable purity. A further ethanol precipitation step can be included but is not necessary if template is simply required for PCR or digestion. This new protocol is helpful for amplification of genes of interest in early-stage research projects and for low throughput screening of transformants. It is more reliable than colony PCR of Synechocystis cultures, and less involved and cheaper than existing clean-DNA preparation methods. It represents an unusually simple and reliable extraction protocol for the growing body of research making use of this cyanobacterium.

Item Type:	Article
Uncontrolled Keywords:	molecular biology, genome, cyanobacteria, Synechocystis
Subjects:	Q Science > QH Natural history > QH301 Biology
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Biology & Biotechnology Research Group Faculty of Engineering & Science > School of Science (SCI)
Last Modified:	02 Oct 2020 09:44
URI:	http://gala.gre.ac.uk/id/eprint/28470

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