Tomlinson, Katie R., Seal, Susan E. ORCID: https://orcid.org/0000-0002-3952-1562, Bailey, Andy M. and Foster, Gary D. ORCID: https://orcid.org/0000-0002-1979-1397 (2019) Utilization of infectious clones to visualize Cassava brown streak virus replication in planta and gain insights into symptom development. Virus Genes, 55 (6). pp. 825-833. ISSN 0920-8569 (Print), 1572-994X (Online) (doi:10.1007/s11262-019-01697-5)

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Abstract

Cassava brown streak disease (CBSD) is a leading cause of cassava yield losses across eastern and central Africa and is having a severe impact on food security across the region. Despite its importance, relatively little is known about the mechanisms behind CBSD viral infections. We have recently reported the construction of Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) infectious clones (IC), which can be used to gain insights into the functions of viral proteins and sequences associated with symptom development. In this study, we perform the first reporter gene tagging of a CBSV IC, with the insertion of green fluorescent protein (GFP) sequence at two different genome positions. Nicotiana benthamiana infections with the CBSV_GFP ICs revealed active CBSV replication in inoculated leaves at 2–5 days post inoculation (dpi) and systemic leaves at 10–14 dpi. We also constructed the chimera CBSV_UCP IC, consisting of the CBSV genome with a UCBSV coat protein (CP) sequence replacement. N. benthamiana infections with CBSV_UCP revealed that the CBSV CP may be associated with high levels of viral accumulation and necrosis development during early infection. These initial manipulations pave the way for U/CBSV ICs to be used to understand U/CBSV biology that will inform vital CBSD control strategies.

Item Type:	Article
Additional Information:	© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Uncontrolled Keywords:	Cassava brown streak virus; Ugandan cassava brown streak virus; Cassava; Food security; Infectious clones
Subjects:	S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified:	29 Nov 2019 15:09
URI:	http://gala.gre.ac.uk/id/eprint/24954

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