Bömer, Moritz ORCID: https://orcid.org/0000-0003-1003-9622, O’Brien, José A., Pérez-Salamó, Imma, Krasauskas, Jovaras, Finch, Paul, Briones, Andrea, Daudi, Arsalan, Souda, Puneet, Tsui, Tjir-Li, Whitelegge, Julian P., Bolwell, G. Paul and Devoto, Alessandra (2018) COI1-dependent jasmonate signalling affects growth, metabolites production and cell wall protein composition in Arabidopsis. Annals of Botany, 122 (7). pp. 1117-1129. ISSN 0305-7364 (Print), 1095-8290 (Online) (doi:10.1093/aob/mcy109)

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Abstract

Background and Aims:
Cultured cell suspensions have been the preferred model to study the apoplast as well as to monitor metabolic and cell cycle-related changes. Previous work showed that methyl jasmonate (MeJA) inhibits leaf growth in a CORONATINE INSENSITIVE 1 (COI1)-dependent manner, with COI1 being the jasmonate (JA) receptor. Here, the effect of COI1 overexpression on the growth of stably transformed arabidopsis cell cultures is described.

Methods:
Time-course experiments were carried out to analyse gene expression, and protein and metabolite levels.

Key Results:
Both MeJA treatment and the overexpression of COI1 modify growth, by altering cell proliferation and expansion. DNA content as well as transcript patterns of cell cycle and cell wall remodelling markers were altered. COI1 overexpression also increases the protein levels of OLIGOGALACTURONIDE OXIDASE 1, BETA-GLUCOSIDASE/ENDOGLUCANASES and POLYGALACTURONASE INHIBITING PROTEIN2, reinforcing the role of COI1 in mediating defence responses and highlighting a link between cell wall loosening and growth regulation. Moreover, changes in the levels of the primary metabolites alanine, serine and succinic acid of MeJA-treated Arabidopsis cell cultures were observed. In addition, COI1 overexpression positively affects the availability of metabolites such as β-alanine, threonic acid, putrescine, glucose and myo-inositol, thereby providing a connection between JA-inhibited growth and stress responses.

Conclusions:
This study contributes to the understanding of the regulation of growth and the production of metabolic resources by JAs and COI1. This will have important implications in dissecting the complex relationships between hormonal and cell wall signalling in plants. The work also provides tools to uncover novel mechanisms co-ordinating cell division and post-mitotic cell expansion in the absence of organ developmental control.

Item Type:	Article
Additional Information:	© The Author(s) 2018. Published by Oxford University Press on behalf of the Annals of Botany Company. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords:	Arabidopsis thaliana, cell suspension culture; COI1, jasmonate, cell cycle, cell wall 25 proteins; primary metabolism; stress–signalling
Subjects:	S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department Faculty of Engineering & Science > Natural Resources Institute > Molecular Virology and Entomology Research Group
Last Modified:	25 Apr 2019 09:16
URI:	http://gala.gre.ac.uk/id/eprint/20469

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