Nkere, Chukwuemeka K., Oyekanmi, Joshua O., Silva, Gonçalo ORCID: 0000-0001-5544-2947 , Bömer, Moritz ORCID: 0000-0003-1003-9622 , Atiri, Gabriel I., Onyeka, Joseph, Maroya, Norbert G., Seal, Susan E. and Kumar, P. Lava (2018) Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP). Archives of Virology, 163 (4). pp. 1057-1061. ISSN 0304-8608 (Print), 1432-8798 (Online) (doi:https://doi.org/10.1007/s00705-018-3706-0)

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Abstract

A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity.

Item Type:	Article
Additional Information:	© The Author(s) 2018. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Uncontrolled Keywords:	closed‑tube reverse transcription loop‑mediated isothermal amplification (CT-RT-LAMP); YMV; Potyvirus; yam; Dioscorea; diagnostic assay
Subjects:	S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department Faculty of Engineering & Science > Natural Resources Institute > Molecular Virology and Entomology Research Group
Last Modified:	15 May 2019 15:19
URI:	http://gala.gre.ac.uk/id/eprint/18879

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