Grzywacz, D. and Moore, S. (2016) Production, formulation, and bioassay of baculoviruses for pest control. In: Lacey, Lawrence A., (ed.) Microbial Control of Insect and Mite Pests from Theory to Practice. Academic Press, Amsterdam, pp. 109-124. ISBN 978-0128035276 (doi:10.1016/B978-0-12-803527-6.00007-X)

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Abstract

The commercial production of baculoviruses for pest control using cultured insect hosts is feasible, and the systems and equipment for achieving this are discussed in this chapter. The rearing and quality systems for ensuring efficient sustainable production are also presented. The production of baculoviruses in the field using wild insects is possible but may only be practical for a few specific baculoviruses. In vitro production in cultured insect cells in bioreactors is possible, but not yet on a commercial scale, and problems in achieving phenotypic and genetic acceptability also need to be resolved. Baculovirus insecticides can be employed as simple aqueous suspensions, but formulation and or tank mixing can significantly improve efficacy, shelf life, and ease of use. The effective use of baculovirus formulations in the field to ensure adequate control and optimal persistence are also covered. Future research and production challenges for expanding the use of baculoviruses are discussed.

Item Type:	Book Section
Uncontrolled Keywords:	Alphabaculovirus; Baculovirus; Betabaculovirus; Field performance; Formulation; Gammabaculovirus; Granulovirus; In vitro; In vivo; Lepidoptera; Nucleopolyhedrovirus; Production; UV protection
Subjects:	S Agriculture > SB Plant culture
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified:	06 Feb 2020 12:51
URI:	http://gala.gre.ac.uk/id/eprint/15998

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