Al-Kuwaiti, N.A., Maruthi, M.N. ORCID: 0000-0002-8060-866X and Seal, S.E. ORCID: 0000-0002-3952-1562 (2016) Molecular characterization of potyviruses infecting potato and vegetables in Iraq. Journal of Plant Pathology: An International Journal of the Italian Phytopathological Society, 98 (3). ISSN 1125-4653 (doi:https://doi.org/10.4454/JPP.V98I3.029)

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Abstract

The genetic diversity of potyviruses infecting potato and vegetables in Iraq was determined by reverse transcription polymerase chain reaction (RT-PCR) targeting the 3´ terminal part of the genome. RT-PCR products were cloned and sequenced. Sequence analysis confirmed the occurrence of potyviruses, namely Bean yellow mosaic virus (BYMV) in broad bean, Potato virus Y (PVY) in potato and tomato, and Zucchini yellow mosaic virus (ZYMV) in zucchini squash. BYMV sequences showed maximum nucleotide sequence identity (97.2-98.7%) with isolates from Australia and Japan. Most PVY sequences were very closely related (ca. 99% nucleotide sequence identity) to PVYO: N isolates from USA and Canada, whereas other sequences showed nucleotide identities (98.5-98.7%) with PVYNTN isolates from Poland, UK and Syria. ZYMV sequences showed maximum nucleotide identity (97.9%) with an isolate from Israel. The low genetic diversity amongst Iraqi potyviruses, alongside their high sequence identities with potyviruses from other countries, suggests a relatively recent introduction to Iraq through imported planting materials.

Item Type:	Article
Uncontrolled Keywords:	potyvirus, PVY, ZYMV, BYMV, Iraq, molecular diversity
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified:	25 Oct 2016 11:36
URI:	http://gala.gre.ac.uk/id/eprint/15591

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