Dyer, Paul D.R., Kotha, Arun K., Pettit, Marie W. and Richardson, Simon C.W. ORCID: https://orcid.org/0000-0002-7927-0649 (2013) Imaging select mammalian organelles using fluorescent microscopy: application to drug delivery. In: Weissig, Volkmar, Elbayoumi, Tamer and Olsen, Mark, (eds.) Methods in Molecular Biology: Cellular and subcellular nanotechnology. Methods in Molecular Biology, 991 . Humana Press, New York, USA, pp. 195-210. ISBN 9781627033350 (doi:10.1007/978-1-62703-336-7)

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Abstract

The microscopic imaging of specific organelles has become a staple of the single-cell assay and has helped define the molecular regulation of many physiological processes. This definition has been made possible by utilizing different criteria to identify specific subpopulations of organelles. These criteria can be biochemical, immunological, or physiological, and in many cases, markers regulate fusion to the organelle they define (e.g., Rab-GTPase proteins). Single-cell imaging technology allows, within the context of drug delivery, an evaluation of the intracellular trafficking of both biological and synthetic macromolecules. However, it should be remembered that there are many limitations associated with this type of study and quantitation is not easy. The temporal dissection of novel and default trafficking of both macromolecular “drugs” and macromolecular drug delivery systems is possible. These methodologies are detailed herein.

Item Type:	Book Section
Additional Information:	[1] Book ISBN: 978-1-62703-335-0 (Print); 978-1-62703-336-7 (Online). [2] Series ISSN: 1064-3745 (Print); 1940-6029 (Online).
Uncontrolled Keywords:	endocytosis, drug delivery, organelle, fluorescent microscopy, live-cell imaging, polymers
Subjects:	R Medicine > RS Pharmacy and materia medica
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Education, Health & Human Sciences > School of Human Sciences (HUM)
Related URLs:	Publisher Publisher Publisher Organisation
Last Modified:	09 Oct 2021 04:46
URI:	http://gala.gre.ac.uk/id/eprint/10612

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