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A sensitive retroviral pseudotype assay for influenza H5N1-neutralizing antibodies

A sensitive retroviral pseudotype assay for influenza H5N1-neutralizing antibodies

Temperton, Nigel J., Hoschler, Katja, Major, Diane, Nicolson, Carolyn, Manvell, Ruth, Hien, Vo Minh, Ha, Do Quang, de Jong, Menno, Zambon, Maria, Takeuchi, Yasuhiro and Weiss, Robin A. (2007) A sensitive retroviral pseudotype assay for influenza H5N1-neutralizing antibodies. Influenza and other respiratory viruses, 1 (3). pp. 105-112. ISSN 1750-2659 (online) (doi:10.1111/j.1750-2659.2007.00016.x)

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Abstract

Background
The World Health Organisation (WHO) recommended the development of simple, safe, sensitive and specific neutralization assays for avian influenza antibodies. We have used retroviral pseudotypes bearing influenza H5 hemagglutinin (HA) as safe, surrogate viruses for influenza neutralization assays which can be carried out at Biosafety Level 2.

Results
Using our assay, sera from patients who had recovered from infection with influenza H5N1, and sera from animals experimentally immunized or infected with H5 tested positive for the presence of neutralizing antibodies to H5N1. Pseudotype neutralizing antibody titers were compared with titers obtained by hemagglutinin inhibition (HI) assays and microneutralization (MN) assays using live virus, and showed a high degree of correlation, sensitivity and specificity.

Conclusions
The pseudotype neutralization assay is as sensitive as horse erythrocyte HI and MN for the detection of antibodies to H5N1. It is safer, and can be applied in a high-throughput format for human and animal surveillance and for the evaluation of vaccines.

Item Type: Article
Additional Information: Article first published online: 26 Jul 2007
Uncontrolled Keywords: H5N1, influenza, pseudotypes, serology, viral neutralization
Subjects: Q Science > QR Microbiology > QR355 Virology
R Medicine > RC Internal medicine
Faculty / Department / Research Group: Faculty of Engineering & Science > Medway School of Pharmacy
Related URLs:
Last Modified: 16 Jul 2012 11:01
URI: http://gala.gre.ac.uk/id/eprint/8563

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