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Type I feline coronavirus spike glycoprotein fails to recognize aminopeptidase N as a functional receptor on feline cell lines

Type I feline coronavirus spike glycoprotein fails to recognize aminopeptidase N as a functional receptor on feline cell lines

Dye, Charlotte, Temperton, Nigel and Siddell, Stuart G. (2007) Type I feline coronavirus spike glycoprotein fails to recognize aminopeptidase N as a functional receptor on feline cell lines. Journal of General Virology, 88 (6). pp. 1753-1760. ISSN 0022-1317 (Print), 1465-2099 (Online) (doi:10.1099/vir.0.82666-0)

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Abstract

There are two types of feline coronaviruses that can be distinguished by serology and sequence analysis. Type I viruses, which are prevalent in the field but are difficult to isolate and propagate in cell culture, and type II viruses, which are less prevalent but replicate well in cell culture. An important determinant of coronavirus infection, in vivo and in cell culture, is the interaction of the virus surface glycoprotein with a cellular receptor. It is generally accepted that feline aminopeptidase N can act as a receptor for the attachment and entry of type II strains, and it has been proposed that the same molecule acts as a receptor for type I viruses. However, the experimental data are inconclusive. The aim of the studies reported here was to provide evidence for or against the involvement of feline aminopeptidase N as a receptor for type I feline coronaviruses. Our approach was to produce retroviral pseudotypes that bear the type I or type II feline coronavirus surface glycoprotein and to screen a range of feline cell lines for the expression of a functional receptor for attachment and entry. Our results show that type I feline coronavirus surface glycoprotein fails to recognize feline aminopeptidase N as a functional receptor on three continuous feline cell lines. This suggests that feline aminopeptidase N is not a receptor for type I feline coronaviruses. Our results also indicate that it should be possible to use retroviral pseudotypes to identify and characterize the cellular receptor for type I feline coronaviruses.

Item Type: Article
Additional Information: [1] Supplementary tables are available with the online version of this paper see: http://vir.sgmjournals.org/content/88/6/1753/suppl/DC1 [2] This is an open access article published by the Society for General Microbiology under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/). [3] Citation: Charlotte Dye, Nigel Temperton, and Stuart G. Siddell Type I feline coronavirus spike glycoprotein fails to recognize aminopeptidase N as a functional receptor on feline cell lines J. Gen. Virol. 2007 88: 1753-1760.
Uncontrolled Keywords: feline aminopeptidase N, type I feline coronaviruses, retroviral pseudotypes, cellular receptor
Subjects: Q Science > QR Microbiology > QR355 Virology
R Medicine > RC Internal medicine
Faculty / Department / Research Group: Faculty of Engineering & Science > Medway School of Pharmacy
Faculty of Engineering & Science
Related URLs:
Last Modified: 05 May 2016 00:34
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/8561

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