Polyunsaturated fatty acids and immune cell functions
Pinto, Eva (2007) Polyunsaturated fatty acids and immune cell functions. PhD thesis, University of Greenwich.Full text not available from this repository.
In MS patients there was a significantly positive relationship between membrane AA and TGF-ß1 indicating that it is the individual long chain (LC) PUFA, i.e. AA that regulate the levels of TGF-ß1. Investigation of the effects of n-6 and n-3 PUFAs on normal healthy PBMC production of TGF-ß1 in vitro showed that PBMC in the presence of phytohaemaglutinin (PHA) supplemented with LA, dihyomo-?-linolenic (DGLA) and arachidonic acid (AA) significantly increase TGF-ß1 compared with non-supplemented PHA-stimulated PBMCs. In contrast, TGF-ß1 levels from PHA-stimulated PBMCs supplemented with a-linolenic (ALA), eicosapentaenoic (EPA) or docosahexaenoic acid (DHA) were significantly decreased suggesting that n-6 fatty acids (LA, DGLA and AA) increase in vitro TGF-ß1 production by PHA-stimulated PBMCs and, in contrast, n-3 fatty acids (ALA, EPA and DHA) decrease TGF-ß1 production.
GLA-rich borage oil supplementation resulted in significantly decreased ex vivo monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1a (MIP-1a) and interleukin-8 (IL-8) production from PHA-or LPS-stimulated PBMC compared with baseline. It also significantly decreased cell surface expression of CD36+, CD54+ and CD62L+ on monocytes. In contrast, there was no association between LA-rich corn oil and these adhesion molecules and chemokines suggesting that GLA and/or its metabolites are affecting the chemokines and adhesion molecules studied.
Overall, results of this study indicate that n-6 long chain PUFAs may have anti-inflammatory properties and might therefore be beneficial in multiple sclerosis, atherosclerosis and other inflammatory diseases.
|Item Type:||Thesis (PhD)|
|Uncontrolled Keywords:||polyunsaturated fatty acid, PUFA, triglyceride, immune system, anti-inflammatory properties, inflammatory disease treatment,|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history
|School / Department / Research Groups:||School of Science
Faculty of Engineering & Science > School of Science
School of Science > Department of Life & Sports Science
Faculty of Engineering & Science > School of Science > Department of Life & Sports Science
|Last Modified:||20 Jun 2012 08:35|
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