Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis
Seal, Susan and Muller, Emanuelle (2007) Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis. Archives of Virology, 152 (4). pp. 819-825. ISSN 0304-8608 (Print) 1432-8798 (Online) (doi:10.1007/s00705-006-0888-7)Full text not available from this repository.
Badnavirus-like particles were observed by ISEM in viral preparations from yam (Dioscorea sansibarensis) leaves from Benin. Use of the viral preparation as template for PCR amplification with badnavirus-specific primers gave rise to a 579-bp product with most nucleotide identity (70.8%) to Dioscorea alata bacilliform virus (DaBV, Accession numbers X94575–X94582), the only other yam badnavirus sequenced to date. A full-length badnavirus sequence was generated, which consisted of 7261 nucleotides with a typical Badnavirus genome organisation. The full-length sequence shared most identity (61.9%) to DaBV (Accession numbers X94575–X94582) and hence represents a member of a new badnavirus species termed Dioscorea sansibarensis bacilliform virus (DsBV).
|Uncontrolled Keywords:||swollen shoot virus, bacilliform virus, alata, expression, dna|
|Subjects:||S Agriculture > S Agriculture (General)|
Q Science > QR Microbiology > QR355 Virology
S Agriculture > SB Plant culture
Q Science > QR Microbiology
|School / Department / Research Groups:||Natural Resources Institute|
Natural Resources Institute > Agriculture, Health & Environment
|Last Modified:||11 Nov 2011 12:06|
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