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Items where Author is "Silva, Goncalo"

Items where Author is "Silva, Goncalo"

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Badna-PCR badnavirus bioinformatics biosecurity Busco cassava brown streak viruses control denaturing gradient gel electrophoresis (DGGE) detection diagnostic diagnostics Dioscorea spp. discovery diversity DNA extraction early virus detection endogenous badnaviral elements Endogenous pararetrovirus epidemiology Episomal badnavirus Fastqc gene flow genetic differentiation genetic variation haplotype high-throughput sequencing Illumina adapters impact isothermal amplification leaf beetle microsatellites mitochondrial DNA multiplex PCR phytopathology plant virus quality control Quast rapid diagnosis Reverse transcriptase recombinase polymerase amplification (RT-RPA) Rolling circle amplification RT-LAMP seed systems surveillance transcriptome assembly Trimmomatic trinity virus virus diagnostics West Africa yam Yam mosaic virus
Number of items: 58.

Badna-PCR

Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu, Rathnayake, Ajith, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Lava Kumar, P. and Seal, Susan ORCID: 0000-0002-3952-1562 (2018) Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes. Bio-protocol, 8 (1). ISSN 2331-8325 (Online) (doi:https://doi.org/10.21769/BioProtoc.2672)

badnavirus

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu A., Nkere, Chukwuemeka K., Kumar, P. Lava and Seal, Susan E. ORCID: 0000-0002-3952-1562 (2022) Homing in on endogenous badnaviral elements: development of multiplex PCR-DGGE for detection and rapid identification of badnavirus sequences in yam germplasm. Frontiers in Plant Science, 13:846989. ISSN 1664-462X (Online) (doi:https://doi.org/10.3389/fpls.2022.846989)

bioinformatics

Kutnjak, Denis, Tamisier, Lucie, Adams, Ian, Boonham, Neil, Candresse, Thierry, Chiumenti, Michela, De Jonghe, Kris, Kreuze, Jan F., Lefebvre, Marie, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Malapi-Wight, Martha, Margaria, Paolo, Mavric Pleško, Irena, McGreig, Sam, Miozzi, Laura, Remenant, Benoit, Reynard, Jean-Sebastien, Rollin, Johan, Rott, Mike, Schumpp, Olivier, Massart, Sébastien and Haegeman, Annelies (2021) A primer on the analysis of high-throughput sequencing data for detection of plant viruses. Microorganisms, 9 (4):841. ISSN 2076-2607 (Online) (doi:https://doi.org/10.3390/microorganisms9040841)

biosecurity

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Tomlinson, Jenny, Onkokesung, Nawaporn, Sommer, Sarah, Mrisho, Latifa, Legg, James, Silva, Ian P., Gutierrez-Vazquez, Yaiza, Howard, Thomas P., Laverick, Alex, Hossain, Oindrila, Wei, Qingshan, M. Gold, Kaitlin and Boonham, Neil (2021) Plant pest surveillance: from satellites to molecules. Emerging Topics in Life Sciences, 5 (2). pp. 275-287. ISSN 2397-8554 (Print), 2397-8562 (Online) (doi:https://doi.org/10.1042/ETLS20200300)

Busco

Sewe, Steven, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Sicat, Juan Paolo, Seal, Susan ORCID: 0000-0002-3952-1562 and Muhindira, Paul Visendi (2022) Trimming and validation of Illumina short reads using Trimmomatic, trinity assembly, and assessment of RNA-Seq Data. In: Edwards, David, (ed.) Plant Bioinformatics. Methods in Molecular Biology. Springer Protocols, 2443 . Humana Press - Springer Nature, New York, United States, pp. 211-232. ISBN 978-1071620663 ; 978-1071620670 (doi:https://doi.org/10.1007/978-1-0716-2067-0_11)

cassava brown streak viruses

Munguti, Florence M., Kilalo, Dora C., Yegon, Hillary K., Macharia, Isaac, Seal, Susan ORCID: 0000-0002-3952-1562 , Mwango’mbe, Agnes W., Nyaboga, Evans N. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2024) Real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of cassava brown streak viruses. Scientific Reports, 14:12438. pp. 1-11. ISSN 2045-2322 (Online) (doi:https://doi.org/10.1038/s41598-024-62249-y)

control

Diouf, Mame Boucar, Festus, Ruth, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Guyader, Sébastien, Umber, Marie, Seal, Susan ORCID: 0000-0002-3952-1562 and Teycheney, Pierre Yves (2022) Viruses of yams (Dioscorea spp.): current gaps in knowledge and future research directions to improve disease management. Viruses, 14 (9):1884. pp. 1-23. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v14091884)

denaturing gradient gel electrophoresis (DGGE)

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu A., Nkere, Chukwuemeka K., Kumar, P. Lava and Seal, Susan E. ORCID: 0000-0002-3952-1562 (2022) Homing in on endogenous badnaviral elements: development of multiplex PCR-DGGE for detection and rapid identification of badnavirus sequences in yam germplasm. Frontiers in Plant Science, 13:846989. ISSN 1664-462X (Online) (doi:https://doi.org/10.3389/fpls.2022.846989)

detection

Kutnjak, Denis, Tamisier, Lucie, Adams, Ian, Boonham, Neil, Candresse, Thierry, Chiumenti, Michela, De Jonghe, Kris, Kreuze, Jan F., Lefebvre, Marie, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Malapi-Wight, Martha, Margaria, Paolo, Mavric Pleško, Irena, McGreig, Sam, Miozzi, Laura, Remenant, Benoit, Reynard, Jean-Sebastien, Rollin, Johan, Rott, Mike, Schumpp, Olivier, Massart, Sébastien and Haegeman, Annelies (2021) A primer on the analysis of high-throughput sequencing data for detection of plant viruses. Microorganisms, 9 (4):841. ISSN 2076-2607 (Online) (doi:https://doi.org/10.3390/microorganisms9040841)

diagnostic

Diouf, Mame Boucar, Festus, Ruth, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Guyader, Sébastien, Umber, Marie, Seal, Susan ORCID: 0000-0002-3952-1562 and Teycheney, Pierre Yves (2022) Viruses of yams (Dioscorea spp.): current gaps in knowledge and future research directions to improve disease management. Viruses, 14 (9):1884. pp. 1-23. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v14091884)

diagnostics

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Tomlinson, Jenny, Onkokesung, Nawaporn, Sommer, Sarah, Mrisho, Latifa, Legg, James, Silva, Ian P., Gutierrez-Vazquez, Yaiza, Howard, Thomas P., Laverick, Alex, Hossain, Oindrila, Wei, Qingshan, M. Gold, Kaitlin and Boonham, Neil (2021) Plant pest surveillance: from satellites to molecules. Emerging Topics in Life Sciences, 5 (2). pp. 275-287. ISSN 2397-8554 (Print), 2397-8562 (Online) (doi:https://doi.org/10.1042/ETLS20200300)

Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu, Rathnayake, Ajith, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Lava Kumar, P. and Seal, Susan ORCID: 0000-0002-3952-1562 (2018) Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes. Bio-protocol, 8 (1). ISSN 2331-8325 (Online) (doi:https://doi.org/10.21769/BioProtoc.2672)

Dioscorea spp.

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu A., Nkere, Chukwuemeka K., Kumar, P. Lava and Seal, Susan E. ORCID: 0000-0002-3952-1562 (2022) Homing in on endogenous badnaviral elements: development of multiplex PCR-DGGE for detection and rapid identification of badnavirus sequences in yam germplasm. Frontiers in Plant Science, 13:846989. ISSN 1664-462X (Online) (doi:https://doi.org/10.3389/fpls.2022.846989)

Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu, Rathnayake, Ajith, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Lava Kumar, P. and Seal, Susan ORCID: 0000-0002-3952-1562 (2018) Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes. Bio-protocol, 8 (1). ISSN 2331-8325 (Online) (doi:https://doi.org/10.21769/BioProtoc.2672)

discovery

Kutnjak, Denis, Tamisier, Lucie, Adams, Ian, Boonham, Neil, Candresse, Thierry, Chiumenti, Michela, De Jonghe, Kris, Kreuze, Jan F., Lefebvre, Marie, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Malapi-Wight, Martha, Margaria, Paolo, Mavric Pleško, Irena, McGreig, Sam, Miozzi, Laura, Remenant, Benoit, Reynard, Jean-Sebastien, Rollin, Johan, Rott, Mike, Schumpp, Olivier, Massart, Sébastien and Haegeman, Annelies (2021) A primer on the analysis of high-throughput sequencing data for detection of plant viruses. Microorganisms, 9 (4):841. ISSN 2076-2607 (Online) (doi:https://doi.org/10.3390/microorganisms9040841)

diversity

Diouf, Mame Boucar, Festus, Ruth, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Guyader, Sébastien, Umber, Marie, Seal, Susan ORCID: 0000-0002-3952-1562 and Teycheney, Pierre Yves (2022) Viruses of yams (Dioscorea spp.): current gaps in knowledge and future research directions to improve disease management. Viruses, 14 (9):1884. pp. 1-23. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v14091884)

DNA extraction

Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu, Rathnayake, Ajith, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Lava Kumar, P. and Seal, Susan ORCID: 0000-0002-3952-1562 (2018) Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes. Bio-protocol, 8 (1). ISSN 2331-8325 (Online) (doi:https://doi.org/10.21769/BioProtoc.2672)

early virus detection

Munguti, Florence M., Kilalo, Dora C., Yegon, Hillary K., Macharia, Isaac, Seal, Susan ORCID: 0000-0002-3952-1562 , Mwango’mbe, Agnes W., Nyaboga, Evans N. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2024) Real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of cassava brown streak viruses. Scientific Reports, 14:12438. pp. 1-11. ISSN 2045-2322 (Online) (doi:https://doi.org/10.1038/s41598-024-62249-y)

endogenous badnaviral elements

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu A., Nkere, Chukwuemeka K., Kumar, P. Lava and Seal, Susan E. ORCID: 0000-0002-3952-1562 (2022) Homing in on endogenous badnaviral elements: development of multiplex PCR-DGGE for detection and rapid identification of badnavirus sequences in yam germplasm. Frontiers in Plant Science, 13:846989. ISSN 1664-462X (Online) (doi:https://doi.org/10.3389/fpls.2022.846989)

Endogenous pararetrovirus

Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu, Rathnayake, Ajith, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Lava Kumar, P. and Seal, Susan ORCID: 0000-0002-3952-1562 (2018) Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes. Bio-protocol, 8 (1). ISSN 2331-8325 (Online) (doi:https://doi.org/10.21769/BioProtoc.2672)

epidemiology

Diouf, Mame Boucar, Festus, Ruth, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Guyader, Sébastien, Umber, Marie, Seal, Susan ORCID: 0000-0002-3952-1562 and Teycheney, Pierre Yves (2022) Viruses of yams (Dioscorea spp.): current gaps in knowledge and future research directions to improve disease management. Viruses, 14 (9):1884. pp. 1-23. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v14091884)

Episomal badnavirus

Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu, Rathnayake, Ajith, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Lava Kumar, P. and Seal, Susan ORCID: 0000-0002-3952-1562 (2018) Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes. Bio-protocol, 8 (1). ISSN 2331-8325 (Online) (doi:https://doi.org/10.21769/BioProtoc.2672)

Fastqc

Sewe, Steven, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Sicat, Juan Paolo, Seal, Susan ORCID: 0000-0002-3952-1562 and Muhindira, Paul Visendi (2022) Trimming and validation of Illumina short reads using Trimmomatic, trinity assembly, and assessment of RNA-Seq Data. In: Edwards, David, (ed.) Plant Bioinformatics. Methods in Molecular Biology. Springer Protocols, 2443 . Humana Press - Springer Nature, New York, United States, pp. 211-232. ISBN 978-1071620663 ; 978-1071620670 (doi:https://doi.org/10.1007/978-1-0716-2067-0_11)

gene flow

Kanyesigye, Dalton, Pius Alibu, Vincent, Tek Tay, Wee, Nalela, Polycarp, Paparu, Pamela, Olaboro, Samuel, Tamusange Nkalubo, Stanley, Siraj Kayondo, Ismail, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Seal, Susan ORCID: 0000-0002-3952-1562 and Hilary Otim, Michael (2022) Population genetic structure of the bean leaf beetle Ootheca mutabilis (Coleoptera: Chrysomelidae) in Uganda. Insects, 13 (6):543. ISSN 2075-4450 (Online) (doi:https://doi.org/10.3390/insects13060543)

genetic differentiation

Kanyesigye, Dalton, Pius Alibu, Vincent, Tek Tay, Wee, Nalela, Polycarp, Paparu, Pamela, Olaboro, Samuel, Tamusange Nkalubo, Stanley, Siraj Kayondo, Ismail, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Seal, Susan ORCID: 0000-0002-3952-1562 and Hilary Otim, Michael (2022) Population genetic structure of the bean leaf beetle Ootheca mutabilis (Coleoptera: Chrysomelidae) in Uganda. Insects, 13 (6):543. ISSN 2075-4450 (Online) (doi:https://doi.org/10.3390/insects13060543)

genetic variation

Kanyesigye, Dalton, Pius Alibu, Vincent, Tek Tay, Wee, Nalela, Polycarp, Paparu, Pamela, Olaboro, Samuel, Tamusange Nkalubo, Stanley, Siraj Kayondo, Ismail, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Seal, Susan ORCID: 0000-0002-3952-1562 and Hilary Otim, Michael (2022) Population genetic structure of the bean leaf beetle Ootheca mutabilis (Coleoptera: Chrysomelidae) in Uganda. Insects, 13 (6):543. ISSN 2075-4450 (Online) (doi:https://doi.org/10.3390/insects13060543)

haplotype

Kanyesigye, Dalton, Pius Alibu, Vincent, Tek Tay, Wee, Nalela, Polycarp, Paparu, Pamela, Olaboro, Samuel, Tamusange Nkalubo, Stanley, Siraj Kayondo, Ismail, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Seal, Susan ORCID: 0000-0002-3952-1562 and Hilary Otim, Michael (2022) Population genetic structure of the bean leaf beetle Ootheca mutabilis (Coleoptera: Chrysomelidae) in Uganda. Insects, 13 (6):543. ISSN 2075-4450 (Online) (doi:https://doi.org/10.3390/insects13060543)

high-throughput sequencing

Kutnjak, Denis, Tamisier, Lucie, Adams, Ian, Boonham, Neil, Candresse, Thierry, Chiumenti, Michela, De Jonghe, Kris, Kreuze, Jan F., Lefebvre, Marie, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Malapi-Wight, Martha, Margaria, Paolo, Mavric Pleško, Irena, McGreig, Sam, Miozzi, Laura, Remenant, Benoit, Reynard, Jean-Sebastien, Rollin, Johan, Rott, Mike, Schumpp, Olivier, Massart, Sébastien and Haegeman, Annelies (2021) A primer on the analysis of high-throughput sequencing data for detection of plant viruses. Microorganisms, 9 (4):841. ISSN 2076-2607 (Online) (doi:https://doi.org/10.3390/microorganisms9040841)

Illumina adapters

Sewe, Steven, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Sicat, Juan Paolo, Seal, Susan ORCID: 0000-0002-3952-1562 and Muhindira, Paul Visendi (2022) Trimming and validation of Illumina short reads using Trimmomatic, trinity assembly, and assessment of RNA-Seq Data. In: Edwards, David, (ed.) Plant Bioinformatics. Methods in Molecular Biology. Springer Protocols, 2443 . Humana Press - Springer Nature, New York, United States, pp. 211-232. ISBN 978-1071620663 ; 978-1071620670 (doi:https://doi.org/10.1007/978-1-0716-2067-0_11)

impact

Diouf, Mame Boucar, Festus, Ruth, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Guyader, Sébastien, Umber, Marie, Seal, Susan ORCID: 0000-0002-3952-1562 and Teycheney, Pierre Yves (2022) Viruses of yams (Dioscorea spp.): current gaps in knowledge and future research directions to improve disease management. Viruses, 14 (9):1884. pp. 1-23. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v14091884)

isothermal amplification

Munguti, Florence M., Kilalo, Dora C., Yegon, Hillary K., Macharia, Isaac, Seal, Susan ORCID: 0000-0002-3952-1562 , Mwango’mbe, Agnes W., Nyaboga, Evans N. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2024) Real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of cassava brown streak viruses. Scientific Reports, 14:12438. pp. 1-11. ISSN 2045-2322 (Online) (doi:https://doi.org/10.1038/s41598-024-62249-y)

Festus, Ruth, Seal, Susan ORCID: 0000-0002-3952-1562 , Prempeh, Ruth, Quain, Marian D. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2023) Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the rapid and sensitive detection of Yam mosaic virus. Viruses, 15 (7):1592. pp. 1-17. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v15071592)

leaf beetle

Kanyesigye, Dalton, Pius Alibu, Vincent, Tek Tay, Wee, Nalela, Polycarp, Paparu, Pamela, Olaboro, Samuel, Tamusange Nkalubo, Stanley, Siraj Kayondo, Ismail, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Seal, Susan ORCID: 0000-0002-3952-1562 and Hilary Otim, Michael (2022) Population genetic structure of the bean leaf beetle Ootheca mutabilis (Coleoptera: Chrysomelidae) in Uganda. Insects, 13 (6):543. ISSN 2075-4450 (Online) (doi:https://doi.org/10.3390/insects13060543)

microsatellites

Kanyesigye, Dalton, Pius Alibu, Vincent, Tek Tay, Wee, Nalela, Polycarp, Paparu, Pamela, Olaboro, Samuel, Tamusange Nkalubo, Stanley, Siraj Kayondo, Ismail, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Seal, Susan ORCID: 0000-0002-3952-1562 and Hilary Otim, Michael (2022) Population genetic structure of the bean leaf beetle Ootheca mutabilis (Coleoptera: Chrysomelidae) in Uganda. Insects, 13 (6):543. ISSN 2075-4450 (Online) (doi:https://doi.org/10.3390/insects13060543)

mitochondrial DNA

Kanyesigye, Dalton, Pius Alibu, Vincent, Tek Tay, Wee, Nalela, Polycarp, Paparu, Pamela, Olaboro, Samuel, Tamusange Nkalubo, Stanley, Siraj Kayondo, Ismail, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Seal, Susan ORCID: 0000-0002-3952-1562 and Hilary Otim, Michael (2022) Population genetic structure of the bean leaf beetle Ootheca mutabilis (Coleoptera: Chrysomelidae) in Uganda. Insects, 13 (6):543. ISSN 2075-4450 (Online) (doi:https://doi.org/10.3390/insects13060543)

multiplex PCR

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu A., Nkere, Chukwuemeka K., Kumar, P. Lava and Seal, Susan E. ORCID: 0000-0002-3952-1562 (2022) Homing in on endogenous badnaviral elements: development of multiplex PCR-DGGE for detection and rapid identification of badnavirus sequences in yam germplasm. Frontiers in Plant Science, 13:846989. ISSN 1664-462X (Online) (doi:https://doi.org/10.3389/fpls.2022.846989)

phytopathology

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Tomlinson, Jenny, Onkokesung, Nawaporn, Sommer, Sarah, Mrisho, Latifa, Legg, James, Silva, Ian P., Gutierrez-Vazquez, Yaiza, Howard, Thomas P., Laverick, Alex, Hossain, Oindrila, Wei, Qingshan, M. Gold, Kaitlin and Boonham, Neil (2021) Plant pest surveillance: from satellites to molecules. Emerging Topics in Life Sciences, 5 (2). pp. 275-287. ISSN 2397-8554 (Print), 2397-8562 (Online) (doi:https://doi.org/10.1042/ETLS20200300)

plant virus

Kutnjak, Denis, Tamisier, Lucie, Adams, Ian, Boonham, Neil, Candresse, Thierry, Chiumenti, Michela, De Jonghe, Kris, Kreuze, Jan F., Lefebvre, Marie, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Malapi-Wight, Martha, Margaria, Paolo, Mavric Pleško, Irena, McGreig, Sam, Miozzi, Laura, Remenant, Benoit, Reynard, Jean-Sebastien, Rollin, Johan, Rott, Mike, Schumpp, Olivier, Massart, Sébastien and Haegeman, Annelies (2021) A primer on the analysis of high-throughput sequencing data for detection of plant viruses. Microorganisms, 9 (4):841. ISSN 2076-2607 (Online) (doi:https://doi.org/10.3390/microorganisms9040841)

quality control

Sewe, Steven, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Sicat, Juan Paolo, Seal, Susan ORCID: 0000-0002-3952-1562 and Muhindira, Paul Visendi (2022) Trimming and validation of Illumina short reads using Trimmomatic, trinity assembly, and assessment of RNA-Seq Data. In: Edwards, David, (ed.) Plant Bioinformatics. Methods in Molecular Biology. Springer Protocols, 2443 . Humana Press - Springer Nature, New York, United States, pp. 211-232. ISBN 978-1071620663 ; 978-1071620670 (doi:https://doi.org/10.1007/978-1-0716-2067-0_11)

Quast

Sewe, Steven, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Sicat, Juan Paolo, Seal, Susan ORCID: 0000-0002-3952-1562 and Muhindira, Paul Visendi (2022) Trimming and validation of Illumina short reads using Trimmomatic, trinity assembly, and assessment of RNA-Seq Data. In: Edwards, David, (ed.) Plant Bioinformatics. Methods in Molecular Biology. Springer Protocols, 2443 . Humana Press - Springer Nature, New York, United States, pp. 211-232. ISBN 978-1071620663 ; 978-1071620670 (doi:https://doi.org/10.1007/978-1-0716-2067-0_11)

rapid diagnosis

Munguti, Florence M., Kilalo, Dora C., Yegon, Hillary K., Macharia, Isaac, Seal, Susan ORCID: 0000-0002-3952-1562 , Mwango’mbe, Agnes W., Nyaboga, Evans N. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2024) Real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of cassava brown streak viruses. Scientific Reports, 14:12438. pp. 1-11. ISSN 2045-2322 (Online) (doi:https://doi.org/10.1038/s41598-024-62249-y)

Reverse transcriptase recombinase polymerase amplification (RT-RPA)

Munguti, Florence M., Kilalo, Dora C., Yegon, Hillary K., Macharia, Isaac, Seal, Susan ORCID: 0000-0002-3952-1562 , Mwango’mbe, Agnes W., Nyaboga, Evans N. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2024) Real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of cassava brown streak viruses. Scientific Reports, 14:12438. pp. 1-11. ISSN 2045-2322 (Online) (doi:https://doi.org/10.1038/s41598-024-62249-y)

Rolling circle amplification

Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu, Rathnayake, Ajith, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Lava Kumar, P. and Seal, Susan ORCID: 0000-0002-3952-1562 (2018) Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes. Bio-protocol, 8 (1). ISSN 2331-8325 (Online) (doi:https://doi.org/10.21769/BioProtoc.2672)

RT-LAMP

Festus, Ruth, Seal, Susan ORCID: 0000-0002-3952-1562 , Prempeh, Ruth, Quain, Marian D. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2023) Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the rapid and sensitive detection of Yam mosaic virus. Viruses, 15 (7):1592. pp. 1-17. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v15071592)

seed systems

Festus, Ruth, Seal, Susan ORCID: 0000-0002-3952-1562 , Prempeh, Ruth, Quain, Marian D. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2023) Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the rapid and sensitive detection of Yam mosaic virus. Viruses, 15 (7):1592. pp. 1-17. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v15071592)

surveillance

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Tomlinson, Jenny, Onkokesung, Nawaporn, Sommer, Sarah, Mrisho, Latifa, Legg, James, Silva, Ian P., Gutierrez-Vazquez, Yaiza, Howard, Thomas P., Laverick, Alex, Hossain, Oindrila, Wei, Qingshan, M. Gold, Kaitlin and Boonham, Neil (2021) Plant pest surveillance: from satellites to molecules. Emerging Topics in Life Sciences, 5 (2). pp. 275-287. ISSN 2397-8554 (Print), 2397-8562 (Online) (doi:https://doi.org/10.1042/ETLS20200300)

transcriptome assembly

Sewe, Steven, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Sicat, Juan Paolo, Seal, Susan ORCID: 0000-0002-3952-1562 and Muhindira, Paul Visendi (2022) Trimming and validation of Illumina short reads using Trimmomatic, trinity assembly, and assessment of RNA-Seq Data. In: Edwards, David, (ed.) Plant Bioinformatics. Methods in Molecular Biology. Springer Protocols, 2443 . Humana Press - Springer Nature, New York, United States, pp. 211-232. ISBN 978-1071620663 ; 978-1071620670 (doi:https://doi.org/10.1007/978-1-0716-2067-0_11)

Trimmomatic

Sewe, Steven, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Sicat, Juan Paolo, Seal, Susan ORCID: 0000-0002-3952-1562 and Muhindira, Paul Visendi (2022) Trimming and validation of Illumina short reads using Trimmomatic, trinity assembly, and assessment of RNA-Seq Data. In: Edwards, David, (ed.) Plant Bioinformatics. Methods in Molecular Biology. Springer Protocols, 2443 . Humana Press - Springer Nature, New York, United States, pp. 211-232. ISBN 978-1071620663 ; 978-1071620670 (doi:https://doi.org/10.1007/978-1-0716-2067-0_11)

trinity

Sewe, Steven, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Sicat, Juan Paolo, Seal, Susan ORCID: 0000-0002-3952-1562 and Muhindira, Paul Visendi (2022) Trimming and validation of Illumina short reads using Trimmomatic, trinity assembly, and assessment of RNA-Seq Data. In: Edwards, David, (ed.) Plant Bioinformatics. Methods in Molecular Biology. Springer Protocols, 2443 . Humana Press - Springer Nature, New York, United States, pp. 211-232. ISBN 978-1071620663 ; 978-1071620670 (doi:https://doi.org/10.1007/978-1-0716-2067-0_11)

virus

Diouf, Mame Boucar, Festus, Ruth, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Guyader, Sébastien, Umber, Marie, Seal, Susan ORCID: 0000-0002-3952-1562 and Teycheney, Pierre Yves (2022) Viruses of yams (Dioscorea spp.): current gaps in knowledge and future research directions to improve disease management. Viruses, 14 (9):1884. pp. 1-23. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v14091884)

virus diagnostics

Festus, Ruth, Seal, Susan ORCID: 0000-0002-3952-1562 , Prempeh, Ruth, Quain, Marian D. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2023) Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the rapid and sensitive detection of Yam mosaic virus. Viruses, 15 (7):1592. pp. 1-17. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v15071592)

West Africa

Festus, Ruth, Seal, Susan ORCID: 0000-0002-3952-1562 , Prempeh, Ruth, Quain, Marian D. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2023) Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the rapid and sensitive detection of Yam mosaic virus. Viruses, 15 (7):1592. pp. 1-17. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v15071592)

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu A., Nkere, Chukwuemeka K., Kumar, P. Lava and Seal, Susan E. ORCID: 0000-0002-3952-1562 (2022) Homing in on endogenous badnaviral elements: development of multiplex PCR-DGGE for detection and rapid identification of badnavirus sequences in yam germplasm. Frontiers in Plant Science, 13:846989. ISSN 1664-462X (Online) (doi:https://doi.org/10.3389/fpls.2022.846989)

yam

Festus, Ruth, Seal, Susan ORCID: 0000-0002-3952-1562 , Prempeh, Ruth, Quain, Marian D. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2023) Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the rapid and sensitive detection of Yam mosaic virus. Viruses, 15 (7):1592. pp. 1-17. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v15071592)

Diouf, Mame Boucar, Festus, Ruth, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Guyader, Sébastien, Umber, Marie, Seal, Susan ORCID: 0000-0002-3952-1562 and Teycheney, Pierre Yves (2022) Viruses of yams (Dioscorea spp.): current gaps in knowledge and future research directions to improve disease management. Viruses, 14 (9):1884. pp. 1-23. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v14091884)

Silva, Goncalo ORCID: 0000-0001-5544-2947 , Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu A., Nkere, Chukwuemeka K., Kumar, P. Lava and Seal, Susan E. ORCID: 0000-0002-3952-1562 (2022) Homing in on endogenous badnaviral elements: development of multiplex PCR-DGGE for detection and rapid identification of badnavirus sequences in yam germplasm. Frontiers in Plant Science, 13:846989. ISSN 1664-462X (Online) (doi:https://doi.org/10.3389/fpls.2022.846989)

Bömer, Moritz ORCID: 0000-0003-1003-9622 , Turaki, Aliyu, Rathnayake, Ajith, Silva, Goncalo ORCID: 0000-0001-5544-2947 , Lava Kumar, P. and Seal, Susan ORCID: 0000-0002-3952-1562 (2018) Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes. Bio-protocol, 8 (1). ISSN 2331-8325 (Online) (doi:https://doi.org/10.21769/BioProtoc.2672)

Yam mosaic virus

Festus, Ruth, Seal, Susan ORCID: 0000-0002-3952-1562 , Prempeh, Ruth, Quain, Marian D. and Silva, Goncalo ORCID: 0000-0001-5544-2947 (2023) Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the rapid and sensitive detection of Yam mosaic virus. Viruses, 15 (7):1592. pp. 1-17. ISSN 1999-4915 (Online) (doi:https://doi.org/10.3390/v15071592)

This list was generated on Thu Nov 21 16:08:45 2024 UTC.