Samir, Haney, Samir, Mohamed ORCID: https://orcid.org/0000-0002-1166-0480, Radwan, Faten, Mandour, Ahmed S., El-Sherbiny, Hossam R., Ahmed, Ahmed Ezzat, Al Syaad, Khalid M., Al-Saeed, Fatimah A. and Watanabe, Gen (2023) Effect of pre-treatment of melatonin on superovulation response, circulatory hormones, and miRNAs in goats during environmental heat stress conditions. Veterinary Research Communications, 48. pp. 459-474. ISSN 0165-7380 (Print), 1573-7446 (Online) (doi:10.1007/s11259-023-10239-2)

Preview

PDF (Author's Accepted Manuscript) 52770 SAMIR_Effect_Of_Pre-Treatment_Of_Melatonin_On_Superovulation_Response_(AAM)_2023.pdf - Accepted Version Download (414kB) | Preview

Abstract

Environmental heat stress has a deleterious impact on farm animal reproductive performance. The purpose of this study was to see how the addition of melatonin affected the efficacy of the superovulation regimen in goats in hot climatic conditions. Sixteen Shiba goats were synchronized and divided into two equal groups (n = 8, each): the melatonin group, which received a single S/C dose of melatonin, and a control group, treated with one ml of corn oil only. Ultrasonographic assessment of ovarian structures (Graafian follicles; GFs and corpus lutea; CLs) morphometry and hemodynamics were performed during the estrous phase of the superovulation (D0) and at day7 after ovulation (D7) of the superovulation regimen. The peripheral reproductive hormones were measured, and microRNAs were characterized. The mean diameter and the total-colored area of GFs during the D0 were significantly (P˂0.05) higher in the melatonin group (5.42 ± 0.11 mm and 1592.20 ± 45.26 pixels, respectively) compared to the control group (4.62 ± 0.12 mm and 1052.55 ± 29.47 pixels, respectively). Concentrations of LH and E2 increased significantly (P˂0.05) in the melatonin group (1.06 ± 0.06 ng/ml and 46.34 ± 2.77 pg/ml, respectively) compared to the control group (0.75 ± 0.12 ng/ml and 29.33 ± 1.89 pg/ml, respectively). At D7, the melatonin-received goats attained greater values in the mean count (6.75 ± 0.33, P˂0.005), diameters (6.08 ± 0.12 mm, P˂0.01), and total-colored area (17137.30 ± 128.53 pixels, P˂0.01) of detected CLs and progesterone concentrations (4.08 ± 0.24 ng/ml) compared to control goats (4.00 ± 0.28, 4.50 ± 0.19 mm, 11156.87 ± 117.90 pixels, and 2.90 ± 0.18 ng/ml respectively). MiRNA expression analysis was identified during both stages denoting several up and downregulated miRNA candidates among the studied groups. In conclusion, incorporating melatonin enhanced the efficiency of the superovulation regimen in goats under hot climatic conditions.

Item Type:	Article
Additional Information:	JSPS postdoctoral fellowship for research in Japan (FY19-ID No. P19101) partially supported this research through the first and last authors.
Uncontrolled Keywords:	Color Doppler ultrasonography, environmental heat stress, goats, melatonin, MiRNAs, reproductive hormones, ovarian hemodynamics, superovulation
Subjects:	G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > Q Science (General) Q Science > QL Zoology
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > School of Science (SCI)
Last Modified:	07 Apr 2026 11:57
URI:	https://gala.gre.ac.uk/id/eprint/52770

Actions (login required)

View Item

Downloads