Worku, Mesfin, Tessema, Belay, Ferede, Getachew, Ochieng, Linnet, Abera Leliso, Shubisa, Mutua, Florence, Moodley, Arshnee, Gelaw, Baye and Grace, Delia ORCID: https://orcid.org/0000-0002-0195-9489 (2025) Prevalence and antimicrobial-resistant Campylobacter jejuni and Campylobacter coli in free-range chickens in Northwest Ethiopia. The American Journal of Tropical Medicine and Hygiene. ISSN 0002-9637 (Print), 1476-1645 (Online) (doi:10.4269/ajtmh.24-0578)

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Abstract

Campylobacter enteritis is the most common bacterial foodborne disease in humans. Long-term use of antibiotics in chicken production may result in antimicrobial resistance in Campylobacter strains. Information on the antimicrobial resistance profile of Campylobacter species among free-range chickens in Ethiopia is scarce. Hence, this study aimed to determine the prevalence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli among free-range chickens in Amhara National Regional state, northwest Ethiopia from November 1, 2022 to April 30, 2023. Cloacal swabs were collected from free-range backyard chickens, directly inoculated onto modified charcoal cefoperazone deoxycholate agar, and incubated at reduced O2 concentration at 42°C for 48 hours. Suspected colonies were confirmed at the species level using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The associated factors were analyzed using the Fisher exact test. A P <0.05 at 95% CI was considered statistically significant. Among the 286 cloacal samples, 15.0% (n = 43/286; CI: 10.2–19.5) were positive for Campylobacter species. C. jejuni (60.5%) was more frequent than C. coli (39.5%). Of the total isolates, 62.8% (n = 27/43), 51.2% (n = 22/43), and 16.3% (n = 7/43) of the Campylobacter species were resistant to tetracycline, ciprofloxacin, and erythromycin, respectively. Of the total Campylobacter species isolates, 9.3% (n = 4/43) were multidrug resistant. Campylobacter species resistance to tetracycline and ciprofloxacin was high in general among backyard chickens. Multidrug-resistant Campylobacter species were also identified, and they require special attention to prevent the potential dissemination of the strains to humans in the community.

Item Type:	Article
Uncontrolled Keywords:	Campylobacter, Ethiopia, antimicrobial resistance
Subjects:	Q Science > Q Science (General) Q Science > QR Microbiology S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Centre for Food Systems Research Faculty of Engineering & Science > Natural Resources Institute > Centre for Food Systems Research > Food Safety and Quality
Last Modified:	01 Jul 2025 15:55
URI:	https://gala.gre.ac.uk/id/eprint/50765

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