Leite, R.F., Gonçalves, J.L., Buanz, Asma ORCID: https://orcid.org/0000-0002-2556-1256, Febraro, C., Craig, D., Van Winden, S., Good, L. and Santos, M.V. (2021) Antimicrobial activity of polyhexamethylene biguanide nanoparticles against mastitis-causing Staphylococcus aureus. JDS Communications, 2 (5). pp. 262-265. ISSN 2666-9102 (Online) (doi:10.3168/jdsc.2021-0114)

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Abstract

Postmilking teat disinfection is one of the main measures used to prevent mastitis caused by contagious pathogens, such as Staphylococcus aureus. The present study evaluated the antimicrobial activity of polyhexamethylene biguanide (PHMB) and PHMB nanoparticles (NP) against mastitis-causing Staph. aureus using a microdilution assay methodology. A total of 20 mastitis-causing Staph. aureus isolates were used to determine the minimum inhibitory concentrations (MIC) of PHMB and PHMB NP compared with 3 disinfectants commonly used for teat disinfection (chlorhexidine digluconate, povidone-iodine, and sodium dichloroisocyanurate). The MIC90 was defined at the concentrations required to inhibit the growth of 90% of Staph. aureus. Our results indicated that PHMB NP presented the lowest MIC value (<0.03 µg/mL) to inhibit 90% of Staph. aureus, followed by chlorhexidine digluconate (⠥0.25 µg/mL) and PHMB (⠥0.5 µg/mL). On the other hand, sodium dichloroisocyanurate (⠥500 µg/mL) and povidone-iodine (⠥8,000 µg/mL) presented the highest concentrations to inhibit the growth of most Staph. aureus. Our preliminary results suggested that both PHMB and PHMB NP have antimicrobial activity against mastitis-causing Staph. aureus, which indicates the potential for both to be used as a teat-dip disinfectant to prevent bovine mastitis.

Item Type:	Article
Additional Information:	Unmapped bibliographic data: DA - 2021/09/01/ [EPrints field already has value set] JO - JDS Communications [Field not mapped to EPrints]
Uncontrolled Keywords:	polyhexamethylene biguanide nanoparticles; antimicrobial activity; Staphylococcus aureus; mastitis
Subjects:	R Medicine > RG Gynecology and obstetrics R Medicine > RS Pharmacy and materia medica
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > School of Science (SCI)
Last Modified:	05 May 2022 10:07
URI:	http://gala.gre.ac.uk/id/eprint/35867

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