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Micro-CT visualization of a promastigote secretory gel (PSG) and parasite plug in the digestive tract of the sand fly Lutzomyia longipalpis infected with Leishmania mexicana

Micro-CT visualization of a promastigote secretory gel (PSG) and parasite plug in the digestive tract of the sand fly Lutzomyia longipalpis infected with Leishmania mexicana

Hall, Martin J. R., Ghosh, Debashi, Martin-Vega, Daniel, Clark, Brett, Clatworthy, Innes, Cheke, Robert A. ORCID: 0000-0002-7437-1934 and Rogers, Matthew E. (2021) Micro-CT visualization of a promastigote secretory gel (PSG) and parasite plug in the digestive tract of the sand fly Lutzomyia longipalpis infected with Leishmania mexicana. PLoS Neglected Tropical Diseases, 15 (8):e0009682. pp. 1-17. ISSN 1935-2735 (Print), 1935-2727 (Online) (doi:https://doi.org/10.1371/journal.pntd.0009682)

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Abstract

Leishmaniasis is a debilitating disease of the tropics, subtropics and southern Europe caused by Leishmania parasites that are transmitted during blood feeding by phlebotomine sand flies (Diptera: Psychodidae). Using non-invasive micro-computed tomography, we were able to visualize the impact of the laboratory model infection of Lutzomyia longipalpis with Leishmania mexicana and its response to a second blood meal. For the first time we were able to show in 3D the plug of promastigote secretory gel (PSG) and parasites in the distended midgut of whole infected sand flies and measure its volume in relation to that of the midgut. We were also able to measure the degree of opening of the stomodeal valve and demonstrate the extension of the PSG and parasites into the pharynx. Although our pilot study could only examine a few flies, it supports the hypothesis that a second, noninfected, blood meal enhances parasite transmission as we showed that the thoracic PSGparasite plug in infected flies after a second blood meal was, on average, more than twice the volume of the plug in infected flies that did not have a second blood meal.

Item Type: Article
Uncontrolled Keywords: Leishmania, sand fly, stomodeal valve, promastigote, secretory gel, parasite plug
Subjects: Q Science > QL Zoology
Q Science > QR Microbiology > QR355 Virology
Faculty / School / Research Centre / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified: 09 Sep 2021 10:08
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
Selected for REF2021: None
URI: http://gala.gre.ac.uk/id/eprint/33706

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