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Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis

Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis

Seal, Susan ORCID: 0000-0002-3952-1562 and Muller, Emanuelle (2007) Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis. Archives of Virology, 152 (4). pp. 819-825. ISSN 0304-8608 (Print), 1432-8798 (Online) (doi:https://doi.org/10.1007/s00705-006-0888-7)

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Abstract

Badnavirus-like particles were observed by ISEM in viral preparations from yam (Dioscorea sansibarensis) leaves from Benin. Use of the viral preparation as template for PCR amplification with badnavirus-specific primers gave rise to a 579-bp product with most nucleotide identity (70.8%) to Dioscorea alata bacilliform virus (DaBV, Accession numbers X94575–X94582), the only other yam badnavirus sequenced to date. A full-length badnavirus sequence was generated, which consisted of 7261 nucleotides with a typical Badnavirus genome organisation. The full-length sequence shared most identity (61.9%) to DaBV (Accession numbers X94575–X94582) and hence represents a member of a new badnavirus species termed Dioscorea sansibarensis bacilliform virus (DsBV).

Item Type: Article
Uncontrolled Keywords: swollen shoot virus, bacilliform virus, alata, expression, dna
Subjects: S Agriculture > S Agriculture (General)
Q Science > QR Microbiology > QR355 Virology
S Agriculture > SB Plant culture
Q Science > QR Microbiology
Faculty / Department / Research Group: Faculty of Engineering & Science > Natural Resources Institute
Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Faculty of Engineering & Science
Related URLs:
Last Modified: 18 Aug 2015 14:35
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
URI: http://gala.gre.ac.uk/id/eprint/2396

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