Skip navigation

Isolation and characterisation of tilapia β-actin promoter and comparison of its activity with carp β-actin promoter

Isolation and characterisation of tilapia β-actin promoter and comparison of its activity with carp β-actin promoter

Rahman, M. Azizur, Hwang, Gyu-Lin, Razak, Shaharudin Abdul, Sohm, Frederic, Farahmand, Hamid, Smith, Alan, Brooks, Carly and Maclean, Norman (2003) Isolation and characterisation of tilapia β-actin promoter and comparison of its activity with carp β-actin promoter. BBA - Biochimica et Biophysica Acta - Gene Structure and Expression, 1625 (1). pp. 11-18. ISSN 0006-3002 (doi:https://doi.org/10.1016/S0167-4781(02)00534-1)

Full text not available from this repository.

Abstract

The regulatory sequence including proximal promoter, untranslated exon 1 and intron 1 of the β-actin gene from tilapia (Oreochromis niloticus) has been isolated and spliced to a β-galactosidase reporter gene to test its activity. Comparisons of promoter activity have been carried out with three different constructs: (1) 1.6 kb tilapia β-actin regulatory sequence, (2) 1.5 kb carp β-actin regulatory sequence, and (3) 4.7 kb carp β-actin regulatory sequence. Although the 1.6 kb tilapia β-actin regulatory sequence gave slightly different expression patterns in tilapia embryos assayed by in situ X-gal staining, no difference was observed in expression level when the tilapia sequence was compared with the 4.7 kb carp β-actin regulatory sequence by quantitative assay. In comparison with the 1.5 kb carp β-actin regulatory sequence, the 1.6 kb tilapia β-actin regulatory sequence gave higher expression levels in tilapia embryos, while a reverse result was observed in zebrafish embryos. In cell transfection experiments, the 1.6 kb tilapia β-actin regulatory sequence showed three to four times better activity in blue gill cells than either the 4.7 kb carp β-actin or the 1.5 kb carp β-actin regulatory sequences. The 1.6 kb tilapia β-actin regulatory sequence also drove higher reporter gene activity in somatic cells of tilapia than did the 4.7 kb carp β-actin regulatory sequence following direct injection of constructs into muscle. Therefore, taken together, the data demonstrate that the tilapia β-actin promoter can be used as an efficient regulatory sequence to produce autotransgenic tilapia.

Item Type: Article
Uncontrolled Keywords: β-actin gene; Promoter; Tilapia; Transgenic fish
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 06 Dec 2016 10:20
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
Selected for GREAT 2019: None
URI: http://gala.gre.ac.uk/id/eprint/14495

Actions (login required)

View Item View Item