Ngwensang, Erica Njeatih, Bouvaine, Sophie ORCID: https://orcid.org/0000-0002-0788-3243 and Maruthi, Midatharahally N. ORCID: https://orcid.org/0000-0002-8060-866X (2024) The transcriptome response of African and South American cassava (Manihot esculenta Crantz) to infection by the begomovirus EACMV-UG. Plant Pathology. ISSN 0032-0862 (Print), 1365-3059 (Online) (In Press)

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Abstract

In this research, we investigated the mechanism of resistance to cassava mosaic begomoviruses (CMBs) that causes cassava mosaic disease (CMD). This is to identify potential new sources of resistance to this economically important disease. Fourteen cassava varieties were tested for their resistance to the severe specie of CMBs: the East African cassava mosaic virus-Uganda (EACMV-UG). While the six African cassava varieties (72-TME 14, TME 204, TZ 130, Nase 1, Nase 3 and Nase 14) expressed no or mild disease symptoms and a contained low viral load and therefore have been classified as resistant, all South American varieties showed severe leaf symptoms and supported high viral load. Nase 3 (resistant), TZ 130 (resistant) and Ebwanateraka (susceptible) were selected and subjected to RNA-Sequencing (RNA-Seq). A time-course transcriptome analysis of the three cassava varieties identified the largest number of differentially expressed genes (DEGs) in Nase 3 (4228) with 1725 genes uniquely overexpressed upon virus infection. Further analyses revealed that resistant varieties responded earlier to the infection than susceptible varieties. Additionally, expressions of heat shock proteins and transcription factors such as ethylene-responsive transcription factor (ERFs), teosinte-like, cycloidea and PCF1 (TCPs), heat stress transcription factor and basic leucine zipper were observed to be upregulated early. Collectively, these results suggest that resistant varieties maintain a low titer of EACMV-UG by activating specific stress-response genes early. The role of these genes in plants remains to be investigated but offer insights into the molecular mechanism of resistance to CMD.

Item Type:	Article
Uncontrolled Keywords:	Cassava, EACMV-UG, RNA sequencing, DEG, resistance
Subjects:	Q Science > Q Science (General) Q Science > QR Microbiology S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health > Plant Disease & Vectors
Last Modified:	16 Dec 2024 12:56
URI:	http://gala.gre.ac.uk/id/eprint/48885

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