Hall, M.J.R., Martin-Vega, D., Clark, B., Ghosh, D., Rogers, M., Pigoli, D., Veriegh, F.B.D., Tetteh-Kumah, A., Osei-Atweneboana, M.Y. and Cheke, R.A. ORCID: https://orcid.org/0000-0002-7437-1934 (2021) Micro-CT imaging of onchocerca infection of simulium damnosum s.l. blackflies and comparison of the peritrophic membrane thickness of forest and savannah flies. Medical and Veterinary Entomology, 35 (3). pp. 231-238. ISSN 0269-283X (Print), 1365-2915 (Online) (doi:10.1111/mve.12509)

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Abstract

Onchocerciasis is a neglected tropical disease (NTD) caused by Onchocerca Diesing 1841 (Spirurida: Onchocercidae) nematodes transmitted by blackflies. It is associated with poverty and imposes a significant health, welfare and economic burden on many tropical countries. Current methods to visualize infections within the vectors rely on invasive methods. However, using micro-computed tomography techniques, without interference from physical tissue manipulation, we visualized in three dimensions for the first time an L1 larva of an Onchocerca species within the thoracic musculature of a blackfly, Simulium damnosum s.l. Theobald 1903 (Diptera:
Simuliidae), naturally infected in Ghana. The possibility that thicker peritrophic membranes in savannah flies could account for their lower parasite loads was not supported, but there were limits to our analysis. While there were no statistically significant differences between the mean thicknesses of the peritrophic membranes,
in the anterior, dorsal and ventral regions, of forest and savannah blackflies killed 34–48min after a blood-meal, the thickness of the peritrophicmembrane in the posterior region could not be measured. Micro-computed tomography has the potential to provide novel information on many other parasite/vector systems and impactful images for public engagement in health education.

Item Type:	Article
Uncontrolled Keywords:	infection, micro-CT, Onchocerca, peritrophic membrane, simulium
Subjects:	S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health Faculty of Engineering & Science > Natural Resources Institute > Centre for Sustainable Agriculture 4 One Health > Chemical Ecology & Plant Biochemistry
Last Modified:	27 Nov 2024 14:34
URI:	http://gala.gre.ac.uk/id/eprint/31010

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