Weinrich, Tobias, Hoh Kam, Jaimie, Ferrara, Bill T. ORCID: 0000-0002-2163-4032 , Thompson, Elinor P. ORCID: 0000-0002-6434-9290 , Mitrofanis, John and Jeffery, Glen (2019) A day in the life of mitochondria reveals shifting workloads. Scientific Reports, 9:13898. ISSN 2045-2322 (Print), 2041-1723 (Online) (doi:https://doi.org/10.1038/s41598-019-48383-y)

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Abstract

Mitochondria provide energy for cellular function. We examine daily changing patterns of mitochondrial function and metabolism in Drosophila in vivo in terms of their complex (I-IV) activity, ATP production, glycolysis, and whole fly respiration in the morning, afternoon and night. Complex activity and respiration showed significant and unexpected variation, peaking in the afternoon. However, ATP levels by contrast are >40% greater in the morning and lowest at night when glycolysis peaks. Complex activity modulation was at the protein level with no evidence for differential transcription over the day. Timing differences between increased ATP production and peaks of complex activity may result from more efficient ATP production early in the day leaving complex activity with spare capacity. Optical stimulation of mitochondria is only possible in the mornings when there is such spare capacity.

These results provide first evidence of shifts in cellular energy capacity at the organism level. Understanding their translation may be significant to the chosen timing of energy demanding interventions to improve function and health.

Item Type:	Article
Uncontrolled Keywords:	organelle biology, mitochondria, circadian, respiration, glycolysis
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Biology & Biotechnology Research Group Faculty of Engineering & Science > School of Science (SCI)
Last Modified:	21 Feb 2023 12:31
URI:	http://gala.gre.ac.uk/id/eprint/22244

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