Buti, M., Sargent, D. J., Bianco, L., Magnago, P., Velasco, R. and Colgan, R. J. ORCID: 0000-0002-0653-5845 (2018) A study of gene expression changes at the Bp-2 locus associated with bitter pit symptom expression in apples (Malus pumila). Molecular Breeding, 38 (7):85. ISSN 1380-3743 (Print), 1572-9788 (Online) (doi:https://doi.org/10.1007/s11032-018-0840-z)

Preview	PDF (Author Accepted Manuscript) 19927 COLGAN_A_Study_of_Gene_Expression_Changes_at_the_Bp-2_Locus_2018.pdf - Accepted Version Download (351kB) | Preview
	PDF (Acceptance Email) 19927 COLGAN_Acceptance_Email_2018.pdf - Additional Metadata Restricted to Repository staff only Download (205kB) | Request a copy
	Image (TIFF) (Figure 1B) 19927 COLGAN_Figure_1B_2018.tif - Draft Version Restricted to Registered users only Download (203kB) | Request a copy
	Image (TIFF) (Figure 2B) 19927 COLGAN_Figure_2B_2018.tif - Supplemental Material Restricted to Registered users only Download (246kB) | Request a copy
	Image (TIFF) (Figure 3B) 19927 COLGAN_Figure_3B_2018.tif - Supplemental Material Restricted to Registered users only Download (1MB) | Request a copy
	Image (TIFF) (Figure 4B) 19927 COLGAN_Figure_4B_2018.tif - Supplemental Material Restricted to Registered users only Download (921kB) | Request a copy
	Image (TIFF) (Figure S1B) 19927 COLGAN_Supplementary_Figure_S1B_2018.tif - Supplemental Material Restricted to Registered users only Download (5MB) | Request a copy
	Image (TIFF) (Figure S2B) 19927 COLGAN_Supplementary_Figure_S2B_2018.tif - Supplemental Material Restricted to Registered users only Download (973kB) | Request a copy

Abstract

Bitter pit is a physiological disorder of apples that develops in the latter stages of fruit development and during storage. It is characterized by localized necrotic cells that collapse and form pits in the epidermis and outer cortex of fruit. The disorder has been associated with low calcium concentrations, and poor calcium distribution within fruit. The mechanism that leads to individual cell necrosis, while surrounding cells remain healthy, is not fully understood. In order to ascertain the underlying process of bitter pit incidence in apple fruit, a mapping population of ‘Braeburn’ (susceptible to bitter pit) × ‘Cameo’ (resistant to bitter pit) was used to map the trait over two growing seasons. A subset of 96 genotypes from the mapping population representing the full range of phenotypes in the same ratio as the full population were selected for genotyping and functional characterization. RNA-Seq analysis on fruits samples of three resistant and three susceptible lines at seven developmental stages (21, 42, 63, 84, 105, 126 and 147 days post fertilization) identified a number of candidate genes displaying differential gene expression. A subset of candidate genes selected based on their position within the identified QTL interval on chromosome 16 validated by RT-qPCR, and two candidate genes displaying differential gene expression were highlighted as strong candidates for the control of bitter pit symptom expression at the Bp-2 locus.

Item Type:	Article
Uncontrolled Keywords:	Apple, QTL, RNAseq, Bitter pit
Subjects:	S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science Faculty of Engineering & Science > Natural Resources Institute Faculty of Engineering & Science > Natural Resources Institute > Food & Markets Department
Last Modified:	30 Apr 2020 14:05
URI:	http://gala.gre.ac.uk/id/eprint/19927

Actions (login required)

View Item

Downloads