Bayer, Philipp E., Ruperao, Pradeep, Mason, Annaliese S., Stiller, Jiri, Chan, Chon-Kit Kenneth, Hayashi, Satomi, Long, Yan, Meng, Jinling, Sutton, Tim, Visendi, Paul, Varshney, Rajeev K., Batley, Jacqueline and Edwards, David (2015) High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus. Theoretical and Applied Genetics, 128 (6). pp. 1039-1047. ISSN 0040-5752 (Print), 1432-2242 (Online) (doi:10.1007/s00122-015-2488-y)

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Abstract

The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene–trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.

Item Type:	Article
Additional Information:	The final publication is available at Springer via http://dx.doi.org/10.1007/s00122-015-2488-y
Uncontrolled Keywords:	Double Haploid; Conversion Processes; Supplementary Table; Recombination Bin; Recombination Map; Genome-wide Recombination; Reference Genome; Reference Cultivar; Reference Genome Sequence; Black Gram Husk; Recombinant Inbred Line; F2 Individual; Heterozygous SNPs; Recombination Hotspot
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified:	12 Oct 2016 14:40
URI:	http://gala.gre.ac.uk/id/eprint/14760

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