Tomlins, K.I., Jewers, K., Coker, R.D. and Nagler, M.J. (1989) A bi-directional HPTLC development method for the detection of low levels of aflatoxin in maize extracts. Chromatographia, 27 (1-2). pp. 49-52. ISSN 0009-5893 (Print), 1612-1112 (Online) (doi:10.1007/BF02290404)

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Abstract

A bi-directional high performance thin layer chromatography (HPTLC) method for the determination of low levels of aflatoxin in extracts of maize has been evaluated. The method, using diethylether and chloroform:xylene:acetone (6∶3∶1) as the two developing solvents was found to have a limit of detection of 0.8 (B1), 0.4 (B2), 1.7 (G1) and 0.4 (G2) μg/kg respectivety, with CVs equal or less than 1.7% (B1), 2.5% (B2), 3.1% (G1), and 3.1% (G2). Inter-plate variation was significantly greater than intra-plate variation.

Compared with two-dimensional HPTLC methods, a 15 to 30 fold improvement in the sample capacity per plate is achieved and positioning errors are much reduced during densitometry.

Item Type:	Article
Uncontrolled Keywords:	thin layer chromatography, bi-directional development, pre-chromatographic clean-up, aflatoxin
Subjects:	S Agriculture > S Agriculture (General)
Faculty / School / Research Centre / Research Group:	Faculty of Engineering & Science > Natural Resources Institute
Related URLs:	Publisher
Last Modified:	06 Aug 2014 14:49
URI:	http://gala.gre.ac.uk/id/eprint/11917

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