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The human Transmembrane Protease Serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes

The human Transmembrane Protease Serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes

Ferrara, Francesca, Molesti, Eleonora, Böttcher-Frieberthäuser, Eva, Cattoli, Giovanni, Corti, Davide, Scott, Simon D. and Temperton, Nigel J. (2013) The human Transmembrane Protease Serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes. Journal of Molecular and Genetic Medicine, 7 (1):55. pp. 309-314. ISSN 1747-0862 (doi:10.4172/1747-0862.1000055)

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Abstract

The monomer of influenza haemagglutinin is synthesized as a single polypeptide precursor that during maturation is cleaved by proteases into two active subunits. Other studies have demonstrated that the human Transmembrane Protease Serine 2 (TMPRSS2) can cleave the HA of human seasonal influenza viruses. Consequently, we have investigated the use of human Transmembrane Protease Serine 2 to produce high titre influenza haemmagglutinin (HA) lentiviral pseudotypes from Group 2 influenza viruses. Such pseudotypes represent powerful and safe tools to study viral entry and immune responses. Influenza pseudotype particles are obtained by co-transfecting human embryonic kidney HEK293T/17 cells using plasmids coding for the influenza HA, HIV gag-pol and a lentiviral vector incorporating firefly luciferase. However, in order to produce Group 2 pseudotypes, it was necessary to co-transfect a plasmid expressing the TMPRSS2 endoprotease, to achieve the necessary HA cleavage for infective particle generation. These lentiviral pseudotypes were shown to transduce HEK293T/17 cells with high efficiency. This demonstrates that TMPRSS2 is necessary for the functional activation, in vitro, of both the HA of human seasonal influenza and other Group 2 HA influenza strains. Additionally, we show that the Group 2 influenza pseudotype particles can be used as surrogate antigens in neutralization assays and are efficiently neutralized by corresponding influenza virus reference sera. These data demonstrate that the viral pseudotype system is a powerful method for serological surveillance of a wide range of influenza viruses.

Item Type: Article
Additional Information: [1] Citation: Ferrara, Francesca, Molesti, Eleonora, Böttcher-Frieberthäuser, Eva, Cattoli, Giovanni, Corti, Davide, Scott, Simon D. and Temperton, Nigel J. (2013) The human Transmembrane Protease Serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes. Journal of Molecular and Genetic Medicine, 7. pp. 309-314. ISSN 1747-0862. [2] Copyright: © Copyright The Author(s). First Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.5). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.
Uncontrolled Keywords: haemagglutinin, protease cleavage, antibody response, pseudotype serology
Subjects: R Medicine > RA Public aspects of medicine
R Medicine > RS Pharmacy and materia medica
Faculty / Department / Research Group: Faculty of Engineering & Science > Medway School of Pharmacy
Related URLs:
Last Modified: 28 Apr 2016 00:21
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/9575

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