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Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes

Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes

Temperton, Nigel J., Chan, Paul K., Simmons, Graham, Zambon, Maria C., Tedder, Richard S., Takeuchi, Yasuhiro and Weiss, Robin A. (2005) Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes. Emerging Infectious Diseases, 11 (3). pp. 411-416. ISSN 1080-6059 (doi:10.3201/eid1103.040906)

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Abstract

The severe acute respiratory syndrome–associated coronavirus (SARS-CoV) spike protein (S) is a major target for neutralizing antibodies. Retroviral SARS-CoV S pseudotypes have been constructed and used to develop an in vitro microneutralization assay that is both sensitive and specific for SARS-CoV neutralizing antibodies. Neutralization titers measured by this assay are highly correlated to those measured by an assay using replication-competent SARS-CoV. No cross-neutralization occurred with human sera known to contain antibodies to coronavirus strains OC43 and 229E. The pseudotype assay was used to profile neutralizing antibody responses against SARS-CoV S in sequential serum samples taken from 41 confirmed SARS patients during the 2003 outbreak in Hong Kong and shows long-lasting immunity in most recovered patients. The pseudotype assay does not require handling live SARS virus; it is a useful tool to determine neutralizing titers during natural infection and the preclinical evaluation of candidate vaccines

Item Type: Article
Additional Information: [1] Emerging Infectious Diseases is a peer reviewed, open access journal [2] Citation: Temperton NJ, Chan PK, Simmons G, Zambon MC, Tedder RS, Takeuchi Y, et al. Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes. Emerg Infect Dis [serial on the Internet]. 2005 Mar [date cited]. http://dx.doi.org/10.3201/eid1103.040906
Uncontrolled Keywords: immunity, pseudotype assay, live SARS virus, neutralizing titers, infection, vaccines
Subjects: Q Science > QR Microbiology > QR355 Virology
R Medicine > RC Internal medicine
Faculty / Department / Research Group: Faculty of Engineering & Science > Medway School of Pharmacy
Related URLs:
Last Modified: 29 Apr 2016 04:47
URI: http://gala.gre.ac.uk/id/eprint/8565

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