Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes
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Temperton, Nigel J., Chan, Paul K., Simmons, Graham, Zambon, Maria C., Tedder, Richard S., Takeuchi, Yasuhiro and Weiss, Robin A. (2005) Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes. Emerging Infectious Diseases, 11 (3). pp. 411-416. ISSN 1080-6059
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Abstract
The severe acute respiratory syndrome–associated coronavirus (SARS-CoV) spike protein (S) is a major target for neutralizing antibodies. Retroviral SARS-CoV S pseudotypes have been constructed and used to develop an in vitro microneutralization assay that is both sensitive and specific for SARS-CoV neutralizing antibodies. Neutralization titers measured by this assay are highly correlated to those measured by an assay using replication-competent SARS-CoV. No cross-neutralization occurred with human sera known to contain antibodies to coronavirus strains OC43 and 229E. The pseudotype assay was used to profile neutralizing antibody responses against SARS-CoV S in sequential serum samples taken from 41 confirmed SARS patients during the 2003 outbreak in Hong Kong and shows long-lasting immunity in most recovered patients. The pseudotype assay does not require handling live SARS virus; it is a useful tool to determine neutralizing titers during natural infection and the preclinical evaluation of candidate vaccines
| Item Type: | Article |
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| Additional Information: | [1] Emerging Infectious Diseases is a peer reviewed, open access journal [2] Citation: Temperton NJ, Chan PK, Simmons G, Zambon MC, Tedder RS, Takeuchi Y, et al. Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes. Emerg Infect Dis [serial on the Internet]. 2005 Mar [date cited]. http://dx.doi.org/10.3201/eid1103.040906 |
| Uncontrolled Keywords: | immunity, pseudotype assay, live SARS virus, neutralizing titers, infection, vaccines |
| Subjects: | Q Science > QR Microbiology > QR355 Virology R Medicine > RC Internal medicine |
| School / Department / Research Groups: | Medway School of Pharmacy |
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| Last Modified: | 16 Jul 2012 12:49 |
| URI: | http://gala.gre.ac.uk/id/eprint/8565 |
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