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Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses

Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses

Oha, Sawyin, Selleck, Paul, Temperton, Nigel J. ORCID: 0000-0002-7978-3815, Chan, Paul K.S., Capecchi, Barbara, Manavis, Jim, Higgins, Geoff, Burrell, Christopher J. and Kok, Tuckweng (2009) Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses. Journal of Virological Methods, 57 (2). pp. 161-167. ISSN 0166-0934 (doi:10.1016/j.jviromet.2008.12.016)

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Abstract

Four IgG1� monoclonal antibodies (mAbs) against Influenza A/Chicken/Vietnam/8/2004 (H5N1) virus are described. Three of these showed neutralizing activities against H5N1 strains from clades 1, 2 and 3 using a retroviral pseudotype or live virus microneutralization assay. In the pseudotype assay, the IC90 neutralizing titre range was >1600–51,200, and with the microneutralization was 80≥10,240. MAb 1C1 showed strong neutralizing activities in both assays. All four mAbs reacted specifically to the immunogen by immunohistochemical staining and to A/Hong Kong/483/1997 (H5N1) and A/Thailand/1(KAN-1)/2004
(H5N1)-infected MDCK cells by immunofluorescence. ELISA titrations of the mAbs showed specificity for H5N1 haemagglutinin (HA) and no cross-reactivity to 15 other Influenza A subtypes. Only mAbs 1C1 and the non-neutralizing 1F7 reacted with HA1, the cleaved subunit of HA, by Western blot. These results suggest that the mAbs recognize distinct or overlapping epitopes and will be useful reagents for construction of specific rapid point-of-care assays or for therapeutic use.

Item Type: Article
Uncontrolled Keywords: [1] These data were presented at the 3rd ESWI conference, Vilamoura, Portugal in September, 2008
Subjects: R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
R Medicine > RC Internal medicine
Faculty / Department / Research Group: Faculty of Engineering & Science > Medway School of Pharmacy
Related URLs:
Last Modified: 13 Jul 2012 14:31
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/8557

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