Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses
Wright, Edward, Hayman, David T.S., Vaughan, Aisling, Temperton, Nigel J., Wood, James L.N., Cunningham, Andrew A., Suu-Ire, Richard, Weiss, Robin A. and Fooks, Anthony R. (2010) Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses. Virology, 408 (2). pp. 183-189. ISSN 0042-6822 (doi:10.1016/j.virol.2010.09.014)Full text not available from this repository.
It is likely that phylogroup 2 lyssaviruses circulate within bat reservoirs. We adapted a pseudotype (pt) neutralisation assay (PNA) to amultiplex format enabling serosurveillance for Lagos bat virus (LBV),Mokola virus (MOKV) and West Caucasian bat virus (WCBV) in a potential reservoir, the African straw-coloured fruit bat, Eidolon helvum. Highly correlated titres were observed between single and multiplex PNAs using ptLBV and ptMOKV (r=0.97, pb0.0001), validating its use for bat serosurveillance. Of the bat serumsamples screened 56% neutralised ptLBV, 27% ptMOKV and 1% ptWCBV. Mean VNAb titres were 1:266, 1:35 and 1:7 against ptLBV, ptMOKV and ptWCBV respectively. The high seroprevalence estimates suggest that the infection rate of LBV in E. helvum remains high enough to persist in this species. This supports the hypothesis that LBV is endemic in Ghanaian E. helvum and we speculate that LBV may have co-evolved with African megachiroptera.
|Additional Information:|| Available online 15 October 2010|
|Uncontrolled Keywords:||lyssavirus, rabies virus, lagos bat virus, pseudotype, bat, Africa, Ghana, antibody, neutralisation, serology|
|Subjects:||R Medicine > RC Internal medicine
R Medicine > RZ Other systems of medicine
|Faculty / Department / Research Groups:||Faculty of Engineering & Science > Medway School of Pharmacy|
|Last Modified:||13 Jul 2012 10:59|
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